Rat anti-Human Nuclear Receptor SHP Monoclonal Antibody | anti-NR0B2 antibody

Nuclear Receptor SHP (NR0B2)

Cat. Num. AAA604269

• Gene Names: NR0B2; SHP; SHP1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified; Purified
• Synonyms: Nuclear Receptor SHP; Monoclonal Antibody; Nuclear Receptor SHP (NR0B2); Anti -Nuclear Receptor SHP (NR0B2); anti-NR0B2 antibody

• Host: Rat
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 6D624
• Specificity: Recognizes human SHP.
• Purity/Purification: Purified; Purified
• Form/Format: Liquid. Purified IgG in PBS, pH 7.4. No preservative added.

• Applicable Applications for anti-NR0B2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA, Western Blotting,; Dilution: ELISA (direct and indirect): 1:5000-1:10,000).; Western Blot: 1:5000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at RT).; Optimal conditions must be determined individually for each application.
• Immunogen: Recombinant human small heterodimer partner (SHP).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of human SHP with MBS604269 (dilution 1:2000)|1: Recombinant human SHP protein (His tagged)|2: HepG2 (#HB-8065) cell lysate.)

Product Categories/Family for anti-NR0B2 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 13259503
• NCBI GeneID: 8431
• NCBI Accession #: NP_068804.1
• NCBI GenBank Nucleotide #: NM_021969.2
• UniProt Accession #: Q15466; Q5QP36
• Molecular Weight: 28,058 Da
• NCBI Official Full Name: nuclear receptor subfamily 0 group B member 2
• NCBI Official Synonym Full Names: nuclear receptor subfamily 0, group B, member 2
• NCBI Official Symbol: NR0B2
• NCBI Official Synonym Symbols: SHP; SHP1
• NCBI Protein Information: nuclear receptor subfamily 0 group B member 2; nuclear receptor SHP; small heterodimer partner; orphan nuclear receptor SHP
• UniProt Protein Name: Nuclear receptor subfamily 0 group B member 2
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR0B2
• UniProt Synonym Gene Names: SHP
• UniProt Entry Name: NR0B2_HUMAN

NCBI Description

The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. [provided by RefSeq, Jul 2008]

Uniprot Description

NR0B2: Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box- containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1. Defects in NR0B2 may be associated with obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the nuclear hormone receptor family. NR0 subfamily.

Protein type: Nuclear receptor co-regulator; Nuclear receptor; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1p36.1

Cellular Component: nucleoplasm; protein complex; cytoplasm; nucleus

Molecular Function: retinoid X receptor binding; protein domain specific binding; peroxisome proliferator activated receptor binding; protein binding; protein homodimerization activity; DNA binding; protein complex binding; steroid hormone receptor activity; thyroid hormone receptor binding; transcription factor activity; transcription corepressor activity

Biological Process: cholesterol metabolic process; transcription initiation from RNA polymerase II promoter; organ regeneration; negative regulation of transcription factor activity; response to glucose stimulus; positive regulation of insulin secretion; steroid hormone mediated signaling; gene expression; negative regulation of transcription from RNA polymerase II promoter

Disease: Obesity