Mouse anti-Human Nuclear Mitotic Apparatus Protein (NuMA) Monoclonal Antibody | anti-NUMA1 antibody

Nuclear Mitotic Apparatus Protein (NuMA) Mouse Monoclonal Antibody

Cat. Num. AAA439176

• Gene Names: NUMA1; NUMA; NMP-22
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: Nuclear Mitotic Apparatus Protein (NuMA); Monoclonal Antibody; Nuclear Mitotic Apparatus Protein (NuMA) Mouse Monoclonal Antibody; Nuclear Mitotic Apparatus Protein 1; NuMA protein; NUMA1; SP-H antigen; Structural nuclear protein;; anti-NUMA1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgM, kappa
• Clone Number: SPM300
• Specificity: Recognizes a phosphorylated protein of 228kDa, identified as nuclear mitotic apparatus protein (NuMA). Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 2101

• Applicable Applications for anti-NUMA1 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml); Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Nuclear
• Immunogen: Colon carcinoma 174T cells
• Hu-Chromosome Location: 11q13
• Positive Control: Exponentially growing any cultured human cells. Tonsil or lymph node.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Tonsil stained with NuMA Monoclonal Antibody (SPM300))

References

Butschak G et al. New monoclonal antibodies recognizing phosphorylated proteins in mitotic cells. Acta Histochem 1995, 97(1):19-31 Compton DA and Cleveland DW. NuMA is required for the proper completion of mitosis. J Cell Biol 1993, 120(4):947-57 Price CM and Pettijohn DE. Redistribution of the nuclear mitotic apparatus protein (NuMA) during mitosis and nuclear assembly. Properties of purified NuMA protein. Exp Cell Res 1986, 166:295-311. Gueth-Hallonet C et al. Cleavage of the nuclear matrix protein NuMA during apoptosis. Exp Cell Res 1997, 233(1):21-24.

NCBI and Uniprot Product Information

• NCBI GI #: 557440899
• NCBI GeneID: 4926
• NCBI Accession #: NP_001273490.1
• NCBI GenBank Nucleotide #: NM_001286561.1
• UniProt Accession #: Q14980; Q14981; Q9BTE9; H0YH75
• Molecular Weight: 228kDa
• NCBI Official Full Name: nuclear mitotic apparatus protein 1 isoform 2
• NCBI Official Synonym Full Names: nuclear mitotic apparatus protein 1
• NCBI Official Symbol: NUMA1
• NCBI Official Synonym Symbols: NUMA; NMP-22
• NCBI Protein Information: nuclear mitotic apparatus protein 1
• UniProt Protein Name: Nuclear mitotic apparatus protein 1
• Protein Family: Nuclear mitotic apparatus protein
• UniProt Gene Name: NUMA1
• UniProt Synonym Gene Names: NMP-22
• UniProt Entry Name: NUMA1_HUMAN

NCBI Description

This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]

Uniprot Description

NuMA-1: a coiled-coil nuclear protein that is required to establish and maintain mammalian spindle poles. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident. Two human splice-variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Cell cycle regulation

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: cell cortex; centrosome; cytoplasm; cytosol; nucleoplasm; nucleus; spindle; spindle microtubule; spindle pole

Molecular Function: protein binding; structural molecule activity

Biological Process: nuclear organization and biogenesis

Disease: Acute Promyelocytic Leukemia