Hamster anti-Rat NOTCH 2 Monoclonal Antibody | anti-NOTCH 2 antibody

HAMSTER ANTI MOUSE NOTCH 2

Cat. Num. AAA219854

• Gene Names: Notch2; N2; AI853703
• Reactivity: Rat
• Applications: Flow Cytometry, Functional Assay
• Synonyms: NOTCH 2; Monoclonal Antibody; HAMSTER ANTI MOUSE NOTCH 2; anti-NOTCH 2 antibody

• Host: Hamster
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: HMN2-35
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 2473

• Applicable Applications for anti-NOTCH 2 antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Immunogen: Mouse Notch 2-Fc fusion protein.; Fusion Partners
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Mouse Notch 2 transfected cells stained with Hamster anti Mouse Notch 2)

Related Product Information for anti-NOTCH 2 antibody

Hamster anti Mouse Notch 2 antibody, clone HMN2-35 specifically recognizes Notch 2, one of the four major transmembrane receptors (Notch 1-4) of the Notch signaling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands. The Notch signaling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic T-cell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta-like protein 1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and the gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signaling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. Signaling through Notch 2 has been implicated in the development of marginal zone B cells (MZB), the sensitization of endothelial cells to apoptosis, and the regulation of the expression of CD23 in B-cell lymphocytic leukemia (B-CLL). Studies have also shown a correlation between a decrease in Notch 2 expression and an increase in grade of human breast cancer. Hamster anti Mouse Notch 2 antibody, clone HMN2-35 has been shown to cross-react with rat mast cell line RBL-2H3 and Y3 myeloma cells, in flow cytometry.

NCBI and Uniprot Product Information

• NCBI GI #: 134288853
• NCBI GeneID: 18129
• NCBI Accession #: NP_035058.2
• NCBI GenBank Nucleotide #: NM_010928.2
• Molecular Weight: 242,663 Da
• NCBI Official Full Name: neurogenic locus notch homolog protein 2
• NCBI Official Synonym Full Names: notch 2
• NCBI Official Symbol: Notch2
• NCBI Official Synonym Symbols: N2; AI853703
• NCBI Protein Information: neurogenic locus notch homolog protein 2; Motch B; Notch gene homolog 2
• UniProt Protein Name: Neurogenic locus notch homolog protein 2
• UniProt Gene Name: Notch2
• UniProt Synonym Gene Names: Notch 2
• UniProt Entry Name: NOTC2_MOUSE

Uniprot Description

Notch 2: a member of the Notch family. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. Affects the implementation of differentiation, proliferation and apoptotic programs. Cleaved in the trans-Golgi network and presented on the cell surface as a heterodimer. This protein functions as a receptor for the membrane bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus.

Protein type: Membrane protein, integral; Receptor, misc.; Motility/polarity/chemotaxis

Cellular Component: cell surface; membrane; integral to plasma membrane; cell; integral to membrane; plasma membrane; nucleus; receptor complex; cilium

Molecular Function: NF-kappaB binding; protein binding; enzyme binding; calcium ion binding

Biological Process: wound healing; positive regulation of apoptosis; positive regulation of osteoclast differentiation; multicellular organismal development; myeloid dendritic cell differentiation; negative regulation of transcription from RNA polymerase II promoter; cell fate determination; bone remodeling; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; morphogenesis of an epithelial sheet; heart looping; cell growth; cell differentiation; cell cycle arrest; positive regulation of BMP signaling pathway; placenta development; regulation of developmental process; embryonic limb morphogenesis; Notch signaling pathway; transcription, DNA-dependent; in utero embryonic development; humoral immune response; organ morphogenesis; inflammatory response to antigenic stimulus; defense response to bacterium; positive regulation of Ras protein signal transduction; determination of left/right symmetry