Mouse anti-Human NME1/nm23-H1/NDPK-A Monoclonal Antibody | anti-NME1 antibody

NME1/nm23-H1/NDPK-A (Suppressor of Metastasis)

Cat. Num. AAA4381120

• Gene Names: NME1; NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: NME1/nm23-H1/NDPK-A; Monoclonal Antibody; NME1/nm23-H1/NDPK-A (Suppressor of Metastasis); AWD; GAAD; Granzyme A activated DNase; Metastasis inhibition factor NM23; NB; NBS; NDP kinase A; NM23 long variant; included; NM23H1B; NME/NM23 nucleoside diphosphate kinase 1; NME1-NME2 spliced read-through transcript; Non-metastatic protein 23; homolog 1; Nucleoside diphosphate kinase A; Tumor metastatic process-associated protein; anti-NME1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: CPTC-NME1-2
• Specificity: The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP-K). In humans, NDP kinase A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human C-Myc promotor.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 152

• Applicable Applications for anti-NME1 antibody: Western Blot (WB)
• Application Notes: WB: 0.5-1.0ug/ml
• Positive Control: HepG2 and HeLa whole cell lysates; heart, brain, placenta and lung carcinoma.
• Immunogen: Recombinant full-length human NME1 protein
• Cellular Localization: Cytoplasmic and Nuclear
• Hu-chromosome Location: 17q21.33
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-Page

(SDS-PAGE Analysis Purified NME1/nm23-H1 Mouse Monoclonal Antibody (CPTC-NME1-2). Confirmation of Purity and Integrity of Antibody.)

References

Steeg, P.S., et al. 1988. J. Natl. Cancer Inst. 80: 200-209

NCBI and Uniprot Product Information

• NCBI GI #: 4557797
• NCBI GeneID: 4830
• NCBI Accession #: NP_000260.1
• NCBI GenBank Nucleotide #: NP_000260.1
• UniProt Accession #: P15531
• Molecular Weight: 23kDa
• NCBI Official Full Name: nucleoside diphosphate kinase A isoform b
• NCBI Official Synonym Full Names: NME/NM23 nucleoside diphosphate kinase 1
• NCBI Official Symbol: NME1
• NCBI Official Synonym Symbols: NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1
• NCBI Protein Information: nucleoside diphosphate kinase A
• UniProt Protein Name: Nucleoside diphosphate kinase A
• Protein Family: Nucleoside diphosphate kinase
• UniProt Gene Name: NME1
• UniProt Synonym Gene Names: NDPKA; NM23; NDK A; NDP kinase A; GAAD
• UniProt Entry Name: NDKA_HUMAN

NCBI Description

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008]

Uniprot Description

NME1: an enzyme with multiple activities including histidine protein kinase, nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase and 3'-5' exonuclease activities. Plays a major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Involved in cell proliferation, differentiation and development, G protein-coupled receptor endocytosis, cytotoxic T cell and NK cell-mediated cell death, and gene expression. Required for neural development including neural patterning and cell fate determination. During granzyme A (GZMA)-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair. Three alternatively spliced human proteins have been reported.

Protein type: Nucleotide Metabolism - purine; Kinase, nucleoside diphosphate; Kinase, other; EC 2.7.4.6; Tumor suppressor; Protein kinase, histidine; Nucleotide Metabolism - pyrimidine; Other group; NDK family

Chromosomal Location of Human Ortholog: 17q21.3

Cellular Component: mitochondrion; membrane; cytoplasm; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; GTP binding; deoxyribonuclease activity; nucleoside diphosphate kinase activity; magnesium ion binding; ATP binding; ribosomal small subunit binding

Biological Process: GTP biosynthetic process; nervous system development; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; UTP biosynthetic process; nucleoside diphosphate phosphorylation; endocytosis; DNA catabolic process; regulation of apoptosis; negative regulation of cell proliferation; CTP biosynthetic process; pyrimidine nucleotide metabolic process; nucleoside triphosphate biosynthetic process; cell differentiation; positive regulation of epithelial cell proliferation; positive regulation of DNA binding; purine nucleotide metabolic process

Disease: Neuroblastoma, Susceptibility To