Mouse Nitrotyrosine Monoclonal Antibody

Nitrotyrosine, mAb 100 ìg 267

Cat. Num. AAA584095

• Applications: Western Blot
• Synonyms: Nitrotyrosine; Monoclonal Antibody; mAb 100 ìg 267; anti-Nitrotyrosine antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered sterile antibody solution in PBS, containing 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)

• Applicable Applications for anti-Nitrotyrosine antibody: F, Immunoasssay (IA), P, Western Blot
• Application Notes: Application Notes: F: also cytospins: acetone fixation 10 min -20 degree C; block endogenous peroxidase by 0.3 % H2O2 in PBS (or methanol for intracellular staining); blocking with 10% NGS or 5 % BSA for 30 min. use at assay dependent concentration; Application Use: For immuno histology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. Warning: keep sterile, do not add sodium azide or any nitric containing preservative since this will affect the antibody!
• Immunogen: Nitrated KLH
• Positive Control: W: mouse kidney lysate, mouse optic nerve, retina, spinal cord and brain lysates, rat aorta lysateP: human lung tissue
• Preparation and Storage: Product should be stored at -20 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-Nitrotyrosine antibody

Product Description: The monoclonal antibody HM.11 recognizes modified amino acid nitrotyrosine in all different species. Nitrotyrosine is formed in tissues in presence of the active metabolite NO and is a stable end product of nitrosylation of tyrosine. Inflammation is characterized by increased nitric oxide (NO) production. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, Amyotrophic Lateral Sclerosis (ALS) and Multiple Sclerosis (MS). Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo and consequently as a marker of NO-mediated tissue damage. The monoclonal antibody HM.11 recognizes nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine

References

1. ter Steege, JCA et al; Nitrotyrosine in plasma of celiac disease patients as detected by a new sandwich ELISA. Free Radic Biol Med 1998, 25: 953 2. Casoni, F et al; Protein nitration in a mouse model of familial amyotrophic lateral sclerosis. J Biol Chem 2005, 280: 16295
3. Kooy, NW et al; Evidence for in vivo peroxynitrite production in human acute lung injury. Am J Respir Crit Care Med 1995, 151: 1250 v 4. Klausz, G et al; Local and peripheral cytokine response and CagA status of Helicobacter pylori-positive patients with duodenal ulcer. Eur Cytokine Netw 2003, 14: 143