Western Blot (WB)
(Western blot detection of Niemann Pick C2 in C6,3T3,Hela cell lysates using Niemann Pick C2 Rabbit mAb(1:1000 diluted). Predicted band size:17kDa. Observed band size:17kDa.)
Rabbit Niemann Pick C2 Monoclonal Antibody | anti-NPC2 antibody
Niemann Pick C2 Rabbit mAb
Gene Names
NPC2; HE1; EDDM1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
Niemann Pick C2; Monoclonal Antibody; Niemann Pick C2 Rabbit mAb; NPC intracellular cholesterol transporter 2; HE1; EDDM1; anti-NPC2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
R09-8D3
Specificity
Detected in gallbladder bile (PubMed:21315718). Detected in fibroblasts, kidney, liver, spleen, small intestine, placenta and testis (at protein level) (PubMed:11125141). Epididymis.
Purity/Purification
Affinity purified
Form/Format
Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applicable Applications for anti-NPC2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000
IHC: 1:500
IHC: 1:500
Immunogen
Recombinant protein of human Niemann Pick C2
Conjugation
Unconjugated
Modification
Unmodification
Subcellular Location
Endoplasmic reticulum, Lysosome, Secreted
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot detection of Niemann Pick C2 in C6,3T3,Hela cell lysates using Niemann Pick C2 Rabbit mAb(1:1000 diluted). Predicted band size:17kDa. Observed band size:17kDa.)
Western Blot (WB)
(Western blot detection of Niemann Pick C2 in C6,3T3,Hela cell lysates using Niemann Pick C2 Rabbit mAb(1:1000 diluted). Predicted band size:17kDa. Observed band size:17kDa.)
Related Product Information for anti-NPC2 antibody
This gene encodes a protein containing a lipid recognition domain. The encoded protein may function in regulating the transport of cholesterol through the late endosomal/lysosomal system. Mutations in this gene have been associated with Niemann-Pick disease, type C2 and frontal lobe atrophy. [provided by RefSeq, Jul 2008]
Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the lysosomal compartment (PubMed:17018531, PubMed:11125141, PubMed:18772377, PubMed:29580834, PubMed:15937921). Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1 (PubMed:17018531, PubMed:18772377, PubMed:27238017). May bind and mobilize cholesterol that is associated with membranes (PubMed:18823126). NPC2 binds cholesterol with a 1:1 stoichiometry (PubMed:17018531). Can bind a variety of sterols, including lathosterol, desmosterol and the plant sterols stigmasterol and beta-sitosterol (PubMed:17018531). The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport.
Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the lysosomal compartment (PubMed:17018531, PubMed:11125141, PubMed:18772377, PubMed:29580834, PubMed:15937921). Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1 (PubMed:17018531, PubMed:18772377, PubMed:27238017). May bind and mobilize cholesterol that is associated with membranes (PubMed:18823126). NPC2 binds cholesterol with a 1:1 stoichiometry (PubMed:17018531). Can bind a variety of sterols, including lathosterol, desmosterol and the plant sterols stigmasterol and beta-sitosterol (PubMed:17018531). The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,416 Da
NCBI Official Full Name
epididymal secretory protein E1
NCBI Official Synonym Full Names
Niemann-Pick disease, type C2
NCBI Official Symbol
NPC2
NCBI Official Synonym Symbols
HE1; EDDM1
NCBI Protein Information
epididymal secretory protein E1; Niemann-Pick disease type C2 protein; epididymal protein 1; human epididymis-specific protein 1; tissue-specific secretory protein
UniProt Protein Name
Epididymal secretory protein E1
Protein Family
UniProt Gene Name
NPC2
UniProt Synonym Gene Names
HE1; He1
UniProt Entry Name
NPC2_HUMAN
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Product Notes
The NPC2 npc2 (Catalog #AAA4752912) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Niemann Pick C2 Rabbit mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Niemann Pick C2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1000 IHC: 1:500. Researchers should empirically determine the suitability of the NPC2 npc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Niemann Pick C2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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