Rat Nidogen/Entactin Monoclonal Antibody | anti-NID-1 antibody

Nidogen/Entactin

Cat. Num. AAA4381567

• Gene Names: Nid1; Nid; entactin; nidogen-1; A630025O17; entactin-1
• Reactivity: Guinea Pig, Mouse; Does not react with Human
• Applications: ELISA
• Purity: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Nidogen/Entactin; Monoclonal Antibody; Nid1; Ent; Entactin 1; NID-1; Nidogen-1; anti-NID-1 antibody

• Host: Rat
• Reactivity: Guinea Pig, Mouse; Does not react with Human
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: ELM1
• Specificity: It reacts with the intact (150kDa) nidogen as well as its proteolytic fragments of 135kDa, 110kDa, 100kDa, and 80kDa. This MAb does not cross-react with other basement membrane components or fibronectin. Entactin/Nidogen is a ubiquitous multi-domain basement membrane protein. Basement membranes are the earliest extracellular matrices produced during embryogenesis. They are synthesized and incorporated into the supramolecular architecture of several components, including laminins, Collagen IV, Nidogen and proteoglycans. Nidogen/Entactin, a sulfated glycoprotein, acts as a link between the extracellular matrix molecules Laminin 1 and Collagen Type IV, and thereby participates in the assembly of basement membranes. Nidogen is synthesized and secreted in primary and established mesenchymal peritubular cells and myoepithelial cells, and it affects adhesion of peritubular cells in an autocrine manner. Nidogen is expressed during embryonic and fetal development exclusively in fully developed basement membranes of the ectoderm and is not expressed in the developing endodermal basement membrane or in membranes disrupted during mesoderm formation.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 1245

• Applicable Applications for anti-NID-1 antibody: ELISA (EIA)
• Application Notes: ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA
• Immunogen: Murine EHS laminin preparation
• Cellular Localization: Basement membrane
• Hu-chromosome Location: 13 A1 (Mouse)
• Positive Control: Mouse kidney or Eye tissue.
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-Page

(SDS-PAGE Analysis Purified Nidogen Mouse Monoclonal Antibody (ELM1). Confirmation of Purity and Integrity of Antibody.)

References

Horiguchi et. al., Arch Dermatol Res, 1989; 281:427-432. | Ljubimov AV et. al., Exp Cell Res, 1986; 165:530-540.

NCBI and Uniprot Product Information

• NCBI GI #: 171543883
• NCBI GeneID: 18073
• NCBI Accession #: NP_035047.2
• NCBI GenBank Nucleotide #: NP_035047.2
• UniProt Accession #: P10493
• Molecular Weight: 105; 135; 110; 100; and 80kDa
• NCBI Official Full Name: nidogen-1
• NCBI Official Synonym Full Names: nidogen 1
• NCBI Official Symbol: Nid1
• NCBI Official Synonym Symbols: Nid; entactin; nidogen-1; A630025O17; entactin-1
• NCBI Protein Information: nidogen-1
• UniProt Protein Name: Nidogen-1
• UniProt Gene Name: Nid1
• UniProt Synonym Gene Names: Ent; NID-1
• UniProt Entry Name: NID1_MOUSE

Uniprot Description

Nidogen-1: Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell- extracellular matrix interactions. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Cellular Component: basal lamina; basement membrane; extracellular matrix; extracellular region; proteinaceous extracellular matrix

Molecular Function: calcium ion binding; collagen binding; extracellular matrix binding; laminin binding; laminin-1 binding; protein binding; proteoglycan binding

Biological Process: cell adhesion; cell-matrix adhesion; extracellular matrix organization and biogenesis; glomerular basement membrane development