NGAL mAb (Mouse anti-human, clone # H11)-Capture Ab Monoclonal Antibody | anti-NGAL antibody

NGAL (Capture Ab)

Cat. Num. AAA355004

• Gene Names: LCN2; 24p3; MSFI; NGAL
• Reactivity: Human
• Applications: Western Blot, Lateral Flow, ELISA
• Purity: Protein A or G purified and supplied in 0.01 M PBS (pH7.4) without preservative.; >95% by HPLC & SDS-PAGE
• Synonyms: NGAL mAb (Mouse anti-human; clone # H11)-Capture Ab; Monoclonal Antibody; NGAL (Capture Ab); Monoclonal Anti- NGAL (Capture Ab); anti-NGAL antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: H11
• Specificity: It specifically recognize the human NGAL.
• Purity/Purification: Protein A or G purified and supplied in 0.01 M PBS (pH7.4) without preservative.; >95% by HPLC & SDS-PAGE
• Form/Format: Liquid
• Concentration: 5 mg/ml (varies by lot)

• Applicable Applications for anti-NGAL antibody: Western Blot (WB), Lateral Flow (LF), ELISA (EIA)
• Application Notes: WB, ELISA, Lateral flow assay (rapid test)
• Immunogen: Recombinant human NGAL.
• Matched Antibody Pairs: Capture Ab: NGAL mAb (clone # H11); Detection Ab: NGAL mAb (clone # 1G2)
• Preparation and Storage: Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles.

Related Product Information for anti-NGAL antibody

Lipocalin-2 (LCN2), also known as oncogene 24p3 or neutrophil gelatinase-associated lipocalin (NGAL), is a protein associated with neutrophil gelatinase. The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. Upon encountering invading bacteria the toll-like receptors on immune cells stimulate the synthesis and secretion of lipocalin-2. Secreted lipocalin-2 then limits bacterial growth by sequestering iron-containing siderophores. Lipocalin-2 also functions as a growth factor.

References

1. Kjeldsen, L., Johnsen, A. H., Sengelov, H., Borregaard, N. Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase. J. Biol. Chem. 268: 10425-10432, 1993. 2. Flo TH, Smith KD, Sato S, Rodriguez DJ, Holmes MA, Strong RK, Akira S, Aderem A (December 2004). "Lipocalin 2 mediates an innate immune response to bacterial infection by sequestrating iron". Nature 432 (7019): 917-21. 3. Schmidt-Ott KM, Mori K, Li JY, Kalandadze A, Cohen DJ, Devarajan P, Barasch J (February 2007). "Dual action of neutrophil gelatinase-associated lipocalin". J. Am. Soc. Nephrol. 18 (2): 407-13.

NCBI and Uniprot Product Information

• NCBI GI #: 38455402
• NCBI GeneID: 3934
• NCBI Accession #: NP_005555.2
• NCBI GenBank Nucleotide #: NM_005564.3
• UniProt Accession #: P80188; P30150; Q5SYV9; Q5SYW0; Q6FGL5; Q92683; A6NII8; B4DWV4; B7ZAA2
• Molecular Weight: 22,588 Da
• NCBI Official Full Name: neutrophil gelatinase-associated lipocalin
• NCBI Official Synonym Full Names: lipocalin 2
• NCBI Official Symbol: LCN2
• NCBI Official Synonym Symbols: 24p3; MSFI; NGAL
• NCBI Protein Information: neutrophil gelatinase-associated lipocalin; p25; lipocalin-2; oncogene 24p3; siderocalin LCN2; migration-stimulating factor inhibitor; 25 kDa alpha-2-microglobulin-related subunit of MMP-9
• UniProt Protein Name: Neutrophil gelatinase-associated lipocalin
• UniProt Gene Name: LCN2
• UniProt Synonym Gene Names: HNL; NGAL; NGAL
• UniProt Entry Name: NGAL_HUMAN

Uniprot Description

Function: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Ref.11

Subunit structure: Homodimer; disulfide-linked. Heterodimer; disulfide-linked with MMP9. Ref.20

Subcellular location: Secreted. Note: Upon binding to the SLC22A17 (24p3R) receptor, it is internalized. Ref.11

Tissue specificity: Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes. Ref.2

Induction: Expression is activated by the oncoprotein BCR-ABL; BCR-ABL misregulates expression via the JAK/STAT pathway and binding of STAT5A to the promoter. Ref.15

Sequence similarities: Belongs to the calycin superfamily. Lipocalin family.

Sequence caution: The sequence CAI13824.1 differs from that shown. Reason: Erroneous gene model prediction.