Rat anti-Mouse Neutrophils Monoclonal Antibody
Neutrophils, Mouse, mAb NIMP-R14, FITC
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Synonyms
Neutrophils; Monoclonal Antibody; Mouse; mAb NIMP-R14; FITC; Lymphocyte antigen 6 complex locus protein G6c; myeloid differentiation antigen Gr1; anti-Neutrophils antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2b
Form/Format
1 ml (100 ug/ml) 0.2 um filtered FITC conjugated antibody solution in PBS, containing 1.0% BSA and 0.02% sodium azide.
Applicable Applications for anti-Neutrophils antibody
F, Flow Cytometry (FC/FACS), FS, Immunoassay (IA), P
Application Notes
Application Notes: IHC-F: Tissue was fixed with acetone (Ref 6) IHC-P: Blocking with 20% normal rabbit serum (Ref 4) FC: 5 x 105 cells were incubated with 10ug/ml antibody (Ref 3) FS: Neutrophil depletion. Mice were treated with NIMP-R14 given intraperitoneally at a dose of 1mg, 6h before infection (Ref 5) Staining for Ly-6G/-6C of frozen section of mouse spleen
Application Use: For flow cytometry and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.
Application Use: For flow cytometry and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.
Conjugate
FITC
Immunogen
Purified BALB/c mouse neutrophils
Positive Control
Mouse neutrophils
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-Neutrophils antibody
Product Description: The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C. The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating pre-monocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses.Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.
References
1. Lopez, A et al; Differentiation antigens on mouse eosinophils and neutrophils identified by monoclonal antibodies, Brit J Haematology 1984, 57: 489
2. McLaren, D et al; Schistosoma mansoni: evidence that immunity in vaccinated and chronically infected CBA/Ca mice is sensitive to treatment with a monoclonal antibody that depletes cutaneous effector cells. Parasite Immunol 1987, 9: 667
3. Van Lent, P et al; Monocytes/macrophages rather than PMN are involved in early cartilage degradation in cationic immune complex arthritits in mice. J Leukoc Biol 1997, 61: 267
4. Lubberts, E et al; Adenoviral vector-mediated overexpression of IL-4 in the knee joint of mice with collagen-induced arthritis prevents cartilage destruction. J Immunol 1999, 163: 4546
5. Tacchini-Cottier, F et al; An immunomodulatory function for neutrophils during the induction of a CD4+ Th2 response in BALB/c mice infected with Leishmania major. J Immunol 2000, 165: 628
6. Vries de, B et al; Complement factor C5a mediates renal ischemia-reperfusion injury independent from neutrophils. J Immunol 2003, 170: 3883
7. Nagendra, S et al; Absence of cross-reactivity between murine Ly-6C and Ly-6G. Cytometry A 2004, 58: 195
8. Navarini, A et al; Innate Immune-induced depletion of bone marrow neutrophils aggravates systemic bacterial infections. PNAS 2009, 106: 7107
2. McLaren, D et al; Schistosoma mansoni: evidence that immunity in vaccinated and chronically infected CBA/Ca mice is sensitive to treatment with a monoclonal antibody that depletes cutaneous effector cells. Parasite Immunol 1987, 9: 667
3. Van Lent, P et al; Monocytes/macrophages rather than PMN are involved in early cartilage degradation in cationic immune complex arthritits in mice. J Leukoc Biol 1997, 61: 267
4. Lubberts, E et al; Adenoviral vector-mediated overexpression of IL-4 in the knee joint of mice with collagen-induced arthritis prevents cartilage destruction. J Immunol 1999, 163: 4546
5. Tacchini-Cottier, F et al; An immunomodulatory function for neutrophils during the induction of a CD4+ Th2 response in BALB/c mice infected with Leishmania major. J Immunol 2000, 165: 628
6. Vries de, B et al; Complement factor C5a mediates renal ischemia-reperfusion injury independent from neutrophils. J Immunol 2003, 170: 3883
7. Nagendra, S et al; Absence of cross-reactivity between murine Ly-6C and Ly-6G. Cytometry A 2004, 58: 195
8. Navarini, A et al; Innate Immune-induced depletion of bone marrow neutrophils aggravates systemic bacterial infections. PNAS 2009, 106: 7107
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Product Notes
The Neutrophils (Catalog #AAA584139) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Neutrophils, Mouse, mAb NIMP-R14, FITC reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Neutrophils can be used in a range of immunoassay formats including, but not limited to, F, Flow Cytometry (FC/FACS), FS, Immunoassay (IA), P. Application Notes: IHC-F: Tissue was fixed with acetone (Ref 6) IHC-P: Blocking with 20% normal rabbit serum (Ref 4) FC: 5 x 105 cells were incubated with 10ug/ml antibody (Ref 3) FS: Neutrophil depletion. Mice were treated with NIMP-R14 given intraperitoneally at a dose of 1mg, 6h before infection (Ref 5) Staining for Ly-6G/-6C of frozen section of mouse spleen Application Use: For flow cytometry and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions. Researchers should empirically determine the suitability of the Neutrophils for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neutrophils, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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