Testing Data #1
(Human Cortical Neurons stained with Mouse anti Human Neuron Specific Enolase (MBS211833))
Mouse anti-Human NEURON SPECIFIC ENOLASE Monoclonal Antibody | anti-ENO2 antibody
MOUSE ANTI HUMAN NEURON SPECIFIC ENOLASE
Gene Names
ENO2; NSE
Reactivity
Human
Applications
Immunohistochemistry, ELISA
Synonyms
NEURON SPECIFIC ENOLASE; Monoclonal Antibody; MOUSE ANTI HUMAN NEURON SPECIFIC ENOLASE; anti-ENO2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
5G10
Specificity
NEURON SPECIFIC ENOLASE
This item is specific for human neuron specific enolase (NSE), a glycolytic enzyme that is expressed in neuronal tissue and is released as a result of damage to neural tissue. An increase in serum levels of NSE has been found in patients with tumours such as neuroblastoma.
This item is specific for human neuron specific enolase (NSE), a glycolytic enzyme that is expressed in neuronal tissue and is released as a result of damage to neural tissue. An increase in serum levels of NSE has been found in patients with tumours such as neuroblastoma.
Form/Format
Purified (Purified IgG - liquid)
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
434
Applicable Applications for anti-ENO2 antibody
Immunohistology - Paraffin (IHC), ELISA (EIA)
Application Notes
Immunohistology - Paraffin: Min Dilution: 1/100
Max Dilution: 1/400
Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Histology Positive Control Tissue: Brain cortex
Max Dilution: 1/400
Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Histology Positive Control Tissue: Brain cortex
Immunogen
Native, from brain
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
Buffer Solution
Phosphate buffered saline
Preparation and Storage
Store at +4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of dispatch.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of dispatch.
Testing Data #1
(Human Cortical Neurons stained with Mouse anti Human Neuron Specific Enolase (MBS211833))
Testing Data #1
(Human Cortical Neurons stained with Mouse anti Human Neuron Specific Enolase (MBS211833))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,269 Da
NCBI Official Full Name
gamma-enolase
NCBI Official Synonym Full Names
enolase 2 (gamma, neuronal)
NCBI Official Symbol
ENO2
NCBI Official Synonym Symbols
NSE
NCBI Protein Information
gamma-enolase; neural enolase; neuron-specific enolase; neurone-specific enolase; neuron specific gamma enolase; 2-phospho-D-glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase
UniProt Protein Name
Gamma-enolase
Protein Family
UniProt Gene Name
ENO2
UniProt Synonym Gene Names
NSE
UniProt Entry Name
ENOG_HUMAN
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Product Notes
The ENO2 eno2 (Catalog #AAA211833) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN NEURON SPECIFIC ENOLASE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NEURON SPECIFIC ENOLASE can be used in a range of immunoassay formats including, but not limited to, Immunohistology - Paraffin (IHC), ELISA (EIA). Immunohistology - Paraffin: Min Dilution: 1/100 Max Dilution: 1/400 Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. Histology Positive Control Tissue: Brain cortex. Researchers should empirically determine the suitability of the ENO2 eno2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEURON SPECIFIC ENOLASE, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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