Mouse Neurofilament M, 160kD Monoclonal Antibody | anti-Nefm antibody

Neurofilament M, 160kD (NF-M)

Cat. Num. AAA606767

• Gene Names: Nefm; Nfm; NF-M; Nef3; NF160; NF165
• Reactivity: Avian, Mammal, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Supernatant; Supernatant
• Synonyms: Neurofilament M; 160kD; Monoclonal Antibody; 160kD (NF-M); Anti -Neurofilament M; anti-Nefm antibody

• Host: Mouse
• Reactivity: Avian, Mammal, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 2Q168
• Specificity: Recognizes an epitope from the last 84 amino acids of rat NF-M (145K). Reacts specifically with NF-M from a variety of mammalian and avian species.
• Purity/Purification: Supernatant; Supernatant
• Form/Format: Supplied as a liquid with no preservatives.

• Applicable Applications for anti-Nefm antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:1000-1:2000; Immunohistochemistry (frozen): 1:50-1:1000 4% PFA fixed tissue sections; Optimal dilutions to be determined by researcher.
• Immunogen: Purified recombinant fusion protein containing the last 168 amino acids of rat NF-M.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-Nefm antibody

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble.

Product Categories/Family for anti-Nefm antibody

Antibodies; Abs to Neurofilament

NCBI and Uniprot Product Information

• NCBI GI #: 112363107
• NCBI GeneID: 18040
• NCBI Accession #: NP_032717.2
• NCBI GenBank Nucleotide #: NM_008691.2
• UniProt Accession #: P08553; Q0VDM8; Q3HRJ6; Q3TNS4; Q3TPK2; Q61961; Q8BQ20; A2VCT5
• Molecular Weight: 95,916 Da
• NCBI Official Full Name: neurofilament medium polypeptide
• NCBI Official Synonym Full Names: neurofilament, medium polypeptide
• NCBI Official Symbol: Nefm
• NCBI Official Synonym Symbols: Nfm; NF-M; Nef3; NF160; NF165
• NCBI Protein Information: neurofilament medium polypeptide; neurofilament-M; OTTMUSP00000017611; neurofilament 3, medium; neurofilament protein M; 160 kDa neurofilament protein; neurofilament triplet M protein
• UniProt Protein Name: Neurofilament medium polypeptide
• Protein Family: Neurofilament medium polypeptide
• UniProt Gene Name: Nefm
• UniProt Synonym Gene Names: Nef3; Nfm
• UniProt Entry Name: NFM_MOUSE

Uniprot Description

NFM: a neurofilament intermediate filament protein. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The levels of phosphorylation are altered developmentally and coincident with a change in the neurofilament function.

Protein type: Cytoskeletal

Cellular Component: neuron projection; cytoskeleton; axon; cell; neurofilament; intermediate filament; intracellular; neuromuscular junction; myelin sheath

Molecular Function: structural molecule activity

Biological Process: neurofilament cytoskeleton organization and biogenesis; axon cargo transport; regulation of axon diameter; intermediate filament cytoskeleton organization and biogenesis; intermediate filament bundle assembly; microtubule cytoskeleton organization and biogenesis