Rat anti-Mouse Netrin G2a Monoclonal Antibody | anti-Ntng2 antibody
Netrin G2a
Gene Names
Ntng2; Lmnt2; 2610016D08Rik; Ntng2
Reactivity
Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Netrin G2a; Monoclonal Antibody; Anti -Netrin G2a; anti-Ntng2 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
7H116
Specificity
Recognizes mouse Netrin G2a in direct ELISAs and Western Blots. Does not crossreact with recombinant mouse Netrin G1a, Netrin 1 or Netrin 4.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Applicable Applications for anti-Ntng2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry.
Dilution: Direct ELISA: 0.5-1ug/ml; The detection limit for recombinant mouse Netrin G2a is ~3ng/well.
Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit for recombinant mouse Netrin-G2a is ~10ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.
Immunohistochemistry: 25ug/ml detects Netrin G2a in frozen normal mouse brain tissue sections.
Dilution: Direct ELISA: 0.5-1ug/ml; The detection limit for recombinant mouse Netrin G2a is ~3ng/well.
Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit for recombinant mouse Netrin-G2a is ~10ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.
Immunohistochemistry: 25ug/ml detects Netrin G2a in frozen normal mouse brain tissue sections.
Immunogen
Recombinant protein corresponding to aa18-504 from mouse Netrin G2a expressed in NSO cells (NP_598007).
Hybridoma
Mouse myeloma with B cells obtained from a rat immunized with purified, NS0-derived, recombinant mouse Netrin G2a.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Ntng2 antibody
Mouse Netrin G2a is a member of a GPI-linked Netrin subfamily that is distantly related to classical Netrins. Netrin G2a is approximately 485aa in length. It contains an N-terminal laminin-related region plus a heparin-binding C-terminus that ends in a GPI-linkage. The laminin-related region shows a globular type VI domain followed by three variable-length EGF-like domains. Alternate splicing generates two larger isoforms. Amino acid insertions of 59aa and 34aa between EGF-like motifs 1 and 2 generate Netrin G2b and Netrin G2c, respectively. Mature mouse Netrin G2a shares 96% and 99% amino acid identity with human Netrin G2 and the equivalent region in rat Netrin G2b, respectively.
Product Categories/Family for anti-Ntng2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
66,166 Da
NCBI Official Full Name
Netrin-G2a
NCBI Official Synonym Full Names
netrin G2
NCBI Official Symbol
Ntng2
NCBI Official Synonym Symbols
Lmnt2; 2610016D08Rik; Ntng2
NCBI Protein Information
netrin-G2; laminet 2; laminet-2; OTTMUSP00000012657; OTTMUSP00000012658
UniProt Protein Name
Netrin-G2
Protein Family
UniProt Gene Name
Ntng2
UniProt Synonym Gene Names
Lmnt2
UniProt Entry Name
NTNG2_MOUSE
NCBI Description
The protein encoded by this gene belongs to a subclass of the netrin family called netrin-G proteins. Unlike classic netrins, which act as diffusible chemoattractants, netrin-Gs are glycosylphosphatidylinositol-anchored membrane proteins that interact with specific transmembrane proteins. In mouse, this gene is preferentially expressed in the cerebral cortex, habenular nucleus and superior colliculus. Knockout mutant mice display a lack of behavioral startle in response to acoustic stimuli. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
Uniprot Description
NTNG2: Involved in controlling patterning and neuronal circuit formation at the laminar, cellular, subcellular and synaptic levels. Promotes neurite outgrowth of both axons and dendrites
Protein type: Cell development/differentiation; Membrane protein, GPI anchor
Cellular Component: anchored to plasma membrane; membrane; axon; plasma membrane
Molecular Function: protein binding
Biological Process: nervous system development; axonogenesis; multicellular organismal development; cell differentiation
Research Articles on Ntng2
1. Mice in which netrin-G2 has been genetically inhibited do not startle to an acoustic stimulus, but otherwise perform normally through a behavioral test battery. There were no inner ear structural abnormalities.
2. Axonal Ntng2 transneuronally determines lamina-specific subdendritic segments.
3. selectively expressed in distinct neuronal subsets that include populations in forebrain, midbrain, hindbrain, spinal cord, and spinal ganglia
2. Axonal Ntng2 transneuronally determines lamina-specific subdendritic segments.
3. selectively expressed in distinct neuronal subsets that include populations in forebrain, midbrain, hindbrain, spinal cord, and spinal ganglia
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Product Notes
The Ntng2 ntng2 (Catalog #AAA600088) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Netrin G2a reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Netrin G2a can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Dilution: Direct ELISA: 0.5-1ug/ml; The detection limit for recombinant mouse Netrin G2a is ~3ng/well. Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit for recombinant mouse Netrin-G2a is ~10ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Immunohistochemistry: 25ug/ml detects Netrin G2a in frozen normal mouse brain tissue sections. Researchers should empirically determine the suitability of the Ntng2 ntng2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Netrin G2a, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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