Rat anti-Mouse Nerve Growth Factor (NGF) Monoclonal Antibody | anti-NGF antibody

Nerve Growth Factor (NGF) Rat Monoclonal Antibody (NGF30)

Cat. Num. AAA588027

• Gene Names: ngfb; ngf; ns:zft386; xx:zft386; zgc:109730
• Reactivity: Mouse
• Applications: Western Blot, Immunofluorescence, ELISA
• Synonyms: Nerve Growth Factor (NGF); Monoclonal Antibody; Nerve Growth Factor (NGF) Rat Monoclonal Antibody (NGF30); Beta-nerve growth factor; Beta-NGF; NGF; anti-NGF antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: lgG2a
• Clone Number: NGF30
• Specificity: This antibody recognizes mouse NGF monomer protein (MW 13 kDa) by WB. It does not cross react with EGF, BSA, APO or insulin.
• Form/Format: Lyophilized protein G purified in PBS pH7.4
• Sequence Length: 194

• Applicable Applications for anti-NGF antibody: Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: If reconstituted in 100 muL: WB: 1:200.
• Immunogen: Polymerized NGF
• Preservative: None
• Preparation and Storage: Lyophilized antibody can be kept at 4 degree C for up to 3 months and should be kept at-20 degree C for long-term storage.; To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).; For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-NGF antibody

Nerve growth factor (NGF) is a small secreted protein which induces the differentiation and survival of particular target neurons. It is perhaps the prototypical growth factor; in that it is one of the first to be described. NGF is critical for the survival and maintenance of sympathetic and sensory neurons. NGF is released from the target cells, binds to and activates its high affinity receptor (TrkA), and is internalized into the responsive neuron. There is some data that shows that NGF can be transported from the axon tip to soma. One of the most important molecules in the nervous system, nerve growth factor and its other neurotrophin family members control the development of the nervous system in the embryo and the maintenance of nervous tissue and neural transmission in the adult.

References

1. Debeir T-A nerve growth factor mimetic TrkA antagonist causes withdrawal of cortical cholinergic boutons in the adult rat. 2. Saragovi HU-A TrkA-selective, fast internalizing nerve growth factor-antibody complex induces trophic but not neuritogenic signals. 3. Kenigsberg RL-Two distinct monoclonal antibodies raised against mouse beta nerve growth factor.

NCBI and Uniprot Product Information

• NCBI GI #: 42409517
• NCBI GeneID: 58133
• NCBI Accession #: NP_954680.1
• NCBI GenBank Nucleotide #: NM_199210.1
• UniProt Accession #: Q6YBR5; Q504K7
• Molecular Weight: 21,903 Da
• NCBI Official Full Name: nerve growth factor
• NCBI Official Synonym Full Names: nerve growth factor b (beta polypeptide)
• NCBI Official Symbol: ngfb
• NCBI Official Synonym Symbols: ngf; ns:zft386; xx:zft386; zgc:109730
• NCBI Protein Information: nerve growth factor
• UniProt Protein Name: Nerve growth factor
• Protein Family: Nerve growth factor
• UniProt Gene Name: ngf
• UniProt Synonym Gene Names: ngfb; NGF; zNGF

Uniprot Description

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons ().