Mouse NEFM / NF-M Monoclonal Antibody | anti-NEFM antibody

Mouse Monoclonal [clone RNF403] (IgG1) to Human NEFM / NF-M

Cat. Num. AAA246564

• Gene Names: NEFM; NFM; NEF3; NF-M
• Reactivity: Xenopus, Hamster, Human, Monkey
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified
• Synonyms: NEFM / NF-M; Monoclonal Antibody; Mouse Monoclonal [clone RNF403] (IgG1) to Human NEFM / NF-M; Anti-NEFM / NF-M Antibody (clone RNF403) IHC-plus; NEFM; 160 kDa neurofilament protein; NEF3; NF-M; NFM; Neurofilament 3; Human NEFM; anti-NEFM antibody

• Host: Mouse
• Reactivity: Xenopus, Hamster, Human, Monkey
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: RNF403
• Specificity: Reacts exclusively with the phosphorylated isoform of the160 kD neurofilament protein.
• Purity/Purification: Purified
• Form/Format: PBS containing 0.09% sodium azide. Sourced in TCS.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 916

• Applicable Applications for anti-NEFM antibody: Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB)
• Application Notes: IHC-Fr (1:50 - 1:100), IHC-P (10 ug/ml), WB (1:100 - 1:500)
• Target Species: Human
• Immunogen Description: Neurofilament preparation of calf brain tissue
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-NEFM / NF-M antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 157738649
• NCBI GeneID: 4741
• NCBI Accession #: NP_005373.2
• NCBI GenBank Nucleotide #: NM_005382.2
• UniProt Accession #: P07197; Q4QRK6; B4DGN2; E9PBF7
• Molecular Weight: 59,468 Da
• NCBI Official Full Name: neurofilament medium polypeptide isoform 1
• NCBI Official Synonym Full Names: neurofilament, medium polypeptide
• NCBI Official Symbol: NEFM
• NCBI Official Synonym Symbols: NFM; NEF3; NF-M
• NCBI Protein Information: neurofilament medium polypeptide; neurofilament 3; 160 kDa neurofilament protein; neurofilament triplet M protein; neurofilament-3 (150 kD medium); neurofilament, medium polypeptide 150kDa
• UniProt Protein Name: Neurofilament medium polypeptide
• Protein Family: Neurofilament medium polypeptide
• UniProt Gene Name: NEFM
• UniProt Synonym Gene Names: NEF3; NFM; NF-M
• UniProt Entry Name: NFM_HUMAN

NCBI Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Uniprot Description

NFM: a neurofilament intermediate filament protein. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The levels of phosphorylation are altered developmentally and coincident with a change in the neurofilament function.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: axon; neurofilament; neuromuscular junction

Molecular Function: protein binding; structural constituent of cytoskeleton; microtubule binding

Biological Process: neurofilament bundle assembly; axon cargo transport; regulation of axon diameter; microtubule cytoskeleton organization and biogenesis