Mouse anti-Mouse, Rat Nav beta 3 Monoclonal Antibody | anti-Nav beta 3 antibody

Mouse Anti-Rat Nav beta 3 Monoclonal IgG2B

Cat. Num. AAA807236

• Gene Names: Scn3b; Scnb3
• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein G Purified
• Synonyms: Nav beta 3; Monoclonal Antibody; Mouse Anti-Rat Nav beta 3 Monoclonal IgG2B; Sodium channel subunit beta-3 Antibody; Scn3b Antibody; KIAA1158 Antibody; Nav beta 3 Antibody: HRP; Mouse Anti-Rat NaVbeta3 Monoclonal; Clone S396-29; Anti-Nav beta 3; Nav beta 3 Antibody; anti-Nav beta 3 antibody

• Host: Mouse
• Reactivity: Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2B
• Clone Number: S396-29
• Purity/Purification: Protein G Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 215

• Applicable Applications for anti-Nav beta 3 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Western Blot: 1:1000
• Immunogen: Fusion protein amino acids 1-215 (full-length) of rat NavBeta3
• Immunogen Species: Rat
• COA: A 1:100 dilution was sufficient for detection of NavBeta3 in 20 ug of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.1% sodium azide
• Conjugate: HRP
• Cellular Localization: Membrane
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western Blot analysis of Mouse Brain showing detection of ~40 kDa Nav Beta 3 protein using Mouse Anti-Nav Beta 3 Monoclonal Antibody, Clone S396-29. Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Primary Antibody: Mouse Anti-Nav Beta 3 Monoclonal Antibody at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~40 kDa.)

Related Product Information for anti-Nav beta 3 antibody

Nav Beta 3 (SCN3B) belongs to the voltage-gated sodium channel group, and modulates channel gating kinetics. It inactivates the sodium channel opening more slowly than its Beta1 subunit. It is also unique in causing persistent sodium currents which are thought to amplify summation of synaptic imputs which is likely to increase the excitability of specific neurons to their individual inputs (2). Its association with neurofascin may target the sodium channels to the nodes of Ranvier of developing axons and retain these channels at the nodes in mature myelinated axons (1, 2). Defects in SCN3B are the cause of Brugada syndrome (3).

Product Categories/Family for anti-Nav beta 3 antibody

Neuroscience; Ion Channels; Sodium Channels; Voltage-Gated Sodium Channels

References

1. Genes and mapped phenotypes. (n.d.). Retrieved March 26, 2015, from http://www.ncbi.nlm.nih.gov/gene/?term=Q9jK00 2. Qu Y., et al. (2001) Mol Cell Neurosci. 18(5): 570-580. 3. Ishikawa T., et al. (2013) Circ J. 77(4): 956-967.

NCBI and Uniprot Product Information

• NCBI GI #: 20806175
• NCBI GeneID: 245956
• NCBI Accession #: NP_620797.1
• NCBI GenBank Nucleotide #: NM_139097.3
• UniProt Accession #: Q9JK00
• Molecular Weight: Detects ~40kDa.
• NCBI Official Full Name: sodium channel subunit beta-3
• NCBI Official Synonym Full Names: sodium voltage-gated channel beta subunit 3
• NCBI Official Symbol: Scn3b
• NCBI Official Synonym Symbols: Scnb3
• NCBI Protein Information: sodium channel subunit beta-3
• UniProt Protein Name: Sodium channel subunit beta-3
• UniProt Gene Name: Scn3b
• UniProt Entry Name: SCN3B_RAT

NCBI Description

voltage-sensitive sodium channel auxiliary subunit; modulates channel gating with distinct kinetics [RGD, Feb 2006]

Uniprot Description

SCN3B: Modulates channel gating kinetics. Causes unique persistent sodium currents. Inactivates the sodium channel opening more slowly than the subunit beta-1. Its association with neurofascin may target the sodium channels to the nodes of Ranvier of developing axons and retain these channels at the nodes in mature myelinated axons. Defects in SCN3B are the cause of Brugada syndrome type 7 (BRGDA7). A tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset. Belongs to the sodium channel auxiliary subunit SCN3B (TC 8.A.17) family.

Protein type: Channel, sodium; Membrane protein, integral

Cellular Component: integral to membrane; plasma membrane; voltage-gated sodium channel complex; Z disc

Molecular Function: sodium channel regulator activity

Biological Process: cardiac muscle contraction; membrane depolarization; nervous system development; positive regulation of heart rate; sensory perception of pain; sodium ion transport