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Western Blot (WB) (Fig-1: Western blot analysis of Napsin. Anti-Napsin antibody (Clone: ABM4H60) was tested at 0.5 ug/ml on K562, Liver and Hek 293 lysates.)

Mouse anti-Human Napsin Monoclonal Antibody | anti-NAPSA antibody

Monoclonal Antibody to Napsin (Clone: ABM4H60)

Gene Names
NAPSA; KAP; Kdap; NAP1; NAPA; SNAPA
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Purified
Protein G Chromatography
Synonyms
Napsin; Monoclonal Antibody; Monoclonal Antibody to Napsin (Clone: ABM4H60); NAPSA; NAP1; NAPA; Aspartyl protease 4; Napsin-1; TA01/TA02; Antibody to Napsin; Anti-Napsin Antibody; Napsin Antibody; anti-NAPSA antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
ABM4H60
Purity/Purification
Purified
Protein G Chromatography
Form/Format
25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Sequence Length
420
Applicable Applications for anti-NAPSA antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 0.5-1 ug/ml
IHC: 5-10 ug/ml
Immunogen Information
A partial length recombinant Napsin protein (amino acids 150-350) was used as the immunogen for this antibody
Antigen
Napsin
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.

Western Blot (WB)

(Fig-1: Western blot analysis of Napsin. Anti-Napsin antibody (Clone: ABM4H60) was tested at 0.5 ug/ml on K562, Liver and Hek 293 lysates.)

Western Blot (WB) (Fig-1: Western blot analysis of Napsin. Anti-Napsin antibody (Clone: ABM4H60) was tested at 0.5 ug/ml on K562, Liver and Hek 293 lysates.)

Immunohistochemistry (IHC)

(Fig-2: Immunohistochemical analysis of Napsin in Lung Adenocarcinoma tissue using Napsin antibody (Clone: ABM4H60) at 5 ug/ml.)

Immunohistochemistry (IHC) (Fig-2: Immunohistochemical analysis of Napsin in Lung Adenocarcinoma tissue using Napsin antibody (Clone: ABM4H60) at 5 ug/ml.)
Related Product Information for anti-NAPSA antibody
Napsin A, a human aspartic proteinase is found primarily in type II pneumocytes and alveolar macrophages and predominantly expressed in lung and kidney. It is a reliable marker for pulmonary adenocarcinoma and is expressed in a subset of ovarian clear cell carcinomas (O-CCCs), endometrial (EM) CCCs, and endometrioid carcinomas (EC). It is also useful in evaluating SH (Sclerosing hemangiomas) (23194051). Napsin A acts as an anti-apoptotic protein that promotes resistance to cisplatin by degradation of the tumor suppressor p53, which is regulator of the cell cycle and co-works with cyclin kinase inhibitors as p21 and p27.
Product Categories/Family for anti-NAPSA antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
45,387 Da
NCBI Official Full Name
Napsin-A
NCBI Official Synonym Full Names
napsin A aspartic peptidase
NCBI Official Symbol
NAPSA
NCBI Official Synonym Symbols
KAP; Kdap; NAP1; NAPA; SNAPA
NCBI Protein Information
napsin-A
UniProt Protein Name
Napsin-A
Protein Family
UniProt Gene Name
NAPSA
UniProt Synonym Gene Names
NAP1; NAPA; ASP4; Asp 4

NCBI Description

This gene encodes a member of the peptidase A1 family of aspartic proteases. The encoded preproprotein is proteolytically processed to generate an activation peptide and the mature protease. The activation peptides of aspartic proteinases function as inhibitors of the protease active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The encoded protease may play a role in the proteolytic processing of pulmonary surfactant protein B in the lung and may function in protein catabolism in the renal proximal tubules. This gene has been described as a marker for lung adenocarcinoma and renal cell carcinoma. [provided by RefSeq, Feb 2016]

Uniprot Description

NAPSA: May be involved in processing of pneumocyte surfactant precursors. Belongs to the peptidase A1 family.

Protein type: EC 3.4.23.-; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: extracellular space; lysosome

Molecular Function: aspartic-type endopeptidase activity; endopeptidase activity; peptidase activity

Biological Process: cellular protein metabolic process; membrane protein proteolysis; protein catabolic process; surfactant homeostasis

Research Articles on NAPSA

Similar Products

Product Notes

The NAPSA napsa (Catalog #AAA668440) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Napsin (Clone: ABM4H60) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Napsin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 0.5-1 ug/ml IHC: 5-10 ug/ml. Researchers should empirically determine the suitability of the NAPSA napsa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Napsin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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