Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NALCN Monoclonal Antibody, Clone S187-7 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NALCN Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) NALCN Antibody. (D) Composite.)
Mouse anti-Mouse, Rat NALCN Monoclonal Antibody | anti-Nalcn antibody
NALCN Antibody: Alkaline Phosphatase
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NALCN Monoclonal Antibody, Clone S187-7 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NALCN Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) NALCN Antibody. (D) Composite.)
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NALCN Monoclonal Antibody, Clone S187-7 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NALCN Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) NALCN Antibody. (D) Composite.)
Western Blot (WB)
(Western Blot analysis of Rat Brain showing detection of ~200 kDa NALCN protein using Mouse Anti-NALCN Monoclonal Antibody, Clone S187-7 . Lane 1: Molecular Weight (MW) Ladder. Lane 3: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-NALCN Monoclonal Antibody at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~200 kDa.)
Western Blot (WB)
(Western Blot analysis of Rat Brain showing detection of ~200 kDa NALCN protein using Mouse Anti-NALCN Monoclonal Antibody, Clone S187-7 . Lane 1: Molecular Weight (MW) Ladder. Lane 3: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-NALCN Monoclonal Antibody at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~200 kDa.)
Scientific Background: NALCN (sodium leak channel non-selective protein), alsoknown as CanIon or VGCNL1 (voltage gated channel-like protein 1), is a 1738 amino acid multi-pass membrane protein that belongs to the cation-nonselective channel family. NALCN is highly conserved in mammals and is widely expressed in the central nervous system. Activated by NK-1R, NALCN is a voltage-independent, nonselective cation channel which is permeable to sodium, potassium and calcium ions. NALCN is responsible for background sodium ion leak conductance in neurons and regulates basal excitability of the nervous systems. Defects of NALCN in mice causes disruption in respiratory rhythm and death occurs within 24 hours of birth. Three isoforms of NALCN exists due to alternative splicing events.
NCBI and Uniprot Product Information
NCBI Description
may act as a voltage activated cation channel [RGD, Feb 2006]
Uniprot Description
VGCNL1: a putative voltage-gated ion channel
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, ion channel; Channel, calcium
Cellular Component: integral to membrane; plasma membrane
Molecular Function: voltage-gated calcium channel activity; voltage-gated ion channel activity; cation channel activity; sodium channel activity
Biological Process: sperm motility
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Product Notes
The Nalcn nalcn (Catalog #AAA802258) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NALCN Antibody: Alkaline Phosphatase reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NALCN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). 1ug/mL (WB). Researchers should empirically determine the suitability of the Nalcn nalcn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NALCN, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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