Rabbit NAK1 Monoclonal Antibody | anti-NR4A1 antibody

NAK1, phosphorylated (Ser351) (NAK-1, Early Response Protein NAK1, GFRP1, HMR, MGC9485, NGFIB, N10, NP10, Nuclear Hormone Receptor NUR/77, Nur77, Nuclear Receptor Subfamily 4 Group A Member 1, NR4A1, Orphan Nuclear Receptor HMR, Orphan Nuclear Receptor TR

Cat. Num. AAA6013588

• Gene Names: NR4A1; HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Purified; Purified
• Synonyms: NAK1; Monoclonal Antibody; phosphorylated (Ser351) (NAK-1; Early Response Protein NAK1; GFRP1; HMR; MGC9485; NGFIB; N10; NP10; Nuclear Hormone Receptor NUR/77; Nur77; Nuclear Receptor Subfamily 4 Group A Member 1; NR4A1; Orphan Nuclear Receptor HMR; Orphan Nuclear Receptor TR; Anti -NAK1; anti-NR4A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: igG
• Clone Number: 11C1052
• Specificity: Recognizes endogenous levels of Nur77, phosphorylated at (Ser351). Species Crossreactivity: mouse, rat.
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-NR4A1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser351 of human Nur77.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of extracts from Jurkat cells, untreated or treated with TPA and calcium ionophore A23187 for the indicated times, using MBS6013588 or Nur77 Rabbit mAb (lower).)

Western Blot (WB)

(Western Blot analysis of extracts from Jurkat cells, untreated or treated with TPA and calcium ionophore A23187 for 4 hours, using MBS6013588. Antibody phospho-specificity was confirmed by pre-incubating the antibody with either a phosphorylated site-specific peptide to block the signal or a non-phosphorylated site-specific peptide that does not block the signal.)

Related Product Information for anti-NR4A1 antibody

Nur77, also known as TR3 and NGFI-B, is an immediate-early response gene and an orphan member of the steroid/thyroid/retinoid receptor superfamily. Nur77 is composed of an amino-terminal transactivation domain, a central DNA-binding domain and a carboxyterminal ligand-binding domain. Expression of Nur77 is rapidly induced by a variety of stimuli, including apoptotic, mitogenic and stress signals. It has been proposed to have many functions related to cell proliferation, differentiation and apoptosis. Nur77 has been extensively studied in T cells where it has been implicated in the process of negative selection and TCR-mediated apoptosis. Nur77 binds to specific DNA elements leading to the regulation of target genes. As a possible mechanism for regulating apoptosis, Nur77 can induce the expression of apoptotic genes such as FasL and TRAIL. Nur77 is heavily phosphorylated by multiple kinases, which may affect its transactivation activity as well as its subcellular localization. Translocation of Nur77 from the nucleus to the mitochondria can regulate its association with Bcl-2 and control the release of cytochrome c, thereby triggering apoptosis. Phosphorylation of Nur77 by Akt or RSK occurs at Ser351 (corresponding to rat Nur77 Ser350 and Ser354 of mouse Nur77), a site within the Nur77 DNA binding domain. Serine phosphorylation at this site can down regulate transcriptional activity of Nur77.

Product Categories/Family for anti-NR4A1 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 127819
• NCBI GeneID: 3164
• UniProt Accession #: P22736; Q15627; B4DML7
• Molecular Weight: 64,463 Da
• NCBI Official Full Name: Nuclear receptor subfamily 4 group A member 1
• NCBI Official Synonym Full Names: nuclear receptor subfamily 4, group A, member 1
• NCBI Official Symbol: NR4A1
• NCBI Official Synonym Symbols: HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77
• NCBI Protein Information: nuclear receptor subfamily 4 group A member 1; ST-59; hormone receptor; TR3 orphan receptor; steroid receptor TR3; testicular receptor 3; early response protein NAK1; orphan nuclear receptor HMR; orphan nuclear receptor TR3; nuclear hormone receptor NUR/77; growth factor-inducible nuclear protein N10; nerve growth factor IB nuclear receptor variant 1
• UniProt Protein Name: Nuclear receptor subfamily 4 group A member 1
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR4A1
• UniProt Synonym Gene Names: GFRP1; HMR; NAK1; Nur77
• UniProt Entry Name: NR4A1_HUMAN

NCBI Description

This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

Nur77: an orphan nuclear receptor and immediate-early gene that regulates cellular proliferation, apoptosis, inflammation, and glucose metabolism. Induced by exercise in muscle and is a functional regulator of glucose metabolism in skeletal muscle. Its level decreases in the muscle of obese insulin-resistant men. Acts concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. A mediator of TCR-directed thymocyte apoptosis. TCR-signaling induces a FAIM/Akt/Nur77 signaling pathway that is critical for modulating apoptosis in developing thymocytes. A physiological substrate of the MEK-ERK-RSK cascade that modulates nuclear export and intracellular translocation during T cell death. Binds DNA as a monomer. Interacts with GADD45GIP1. Overexpression of Nur77 induces the expression of both p300 and HDAC1. Acetylation by p300 and HDAC1 may regulate the rapid turnover of Nur77 protein.

Protein type: Nuclear receptor; Apoptosis; DNA-binding

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity

Biological Process: epidermal growth factor receptor signaling pathway; transcription initiation from RNA polymerase II promoter; phosphoinositide-mediated signaling; intracellular receptor-mediated signaling pathway; fibroblast growth factor receptor signaling pathway; cell migration during sprouting angiogenesis; nerve growth factor receptor signaling pathway; innate immune response; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; signal transduction