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Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Rhabdomyosarcoma. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
Mouse anti-Human Myogenin Monoclonal Antibody | anti-MYOG antibody
Myogenin (3G9) Mouse mAb
Gene Names
MYOG; MYF4; myf-4; bHLHc3
Reactivity
Human
Applications
Immunohistochemistry
Purity
Affinity Chromatography
Synonyms
Myogenin; Monoclonal Antibody; Myogenin (3G9) Mouse mAb; myogenin; MYF4; myf-4; BHLHC3; anti-MYOG antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1, kappa
Clone Number
3G9-3A9-2B3
Specificity
This antibody detects endogenous levels of human Myogenin
Purity/Purification
Affinity Chromatography
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-MYOG antibody
Immunohistochemistry-Paraffin (IHC-P)
Application Notes
IHC-P: 1:100-1:500
Immunogen
Synthesized peptide derived from human Myogenin
Conjugation
Unconjugated
Modification
Unmodified
Cell Position
Nucleus
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Rhabdomyosarcoma. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Rhabdomyosarcoma. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Rhabdomyosarcoma. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Rhabdomyosarcoma. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Rhabdomyosarcoma. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Rhabdomyosarcoma. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Rhabdomyosarcoma. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Rhabdomyosarcoma. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
Related Product Information for anti-MYOG antibody
Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts.
Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. [provided by RefSeq, Jul 2008]
Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. [provided by RefSeq, Jul 2008]
Product Categories/Family for anti-MYOG antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25kDa
NCBI Official Full Name
myogenin
NCBI Official Synonym Full Names
myogenin (myogenic factor 4)
NCBI Official Symbol
MYOG
NCBI Official Synonym Symbols
MYF4; myf-4; bHLHc3
NCBI Protein Information
myogenin; myogenic factor 4; class C basic helix-loop-helix protein 3
UniProt Protein Name
Myogenin
Protein Family
UniProt Gene Name
MYOG
UniProt Synonym Gene Names
BHLHC3; MYF4; bHLHc3; Myf-4
UniProt Entry Name
MYOG_HUMAN
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Product Notes
The MYOG myog (Catalog #AAA4753909) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Myogenin (3G9) Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Myogenin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P). IHC-P: 1:100-1:500. Researchers should empirically determine the suitability of the MYOG myog for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myogenin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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