Mouse MyoD1 Monoclonal Antibody | anti-MyoD1 antibody

MyoD1 (bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM) (HRP)

Cat. Num. AAA6123519

• Gene Names: MYOD1; PUM; MYF3; MYOD; bHLHc1
• Reactivity: Chicken, Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: MyoD1; Monoclonal Antibody; MyoD1 (bHLHc1; Class C basic helix-loop-helix protein 1; Myoblast determination protein 1; Myogenic differentiation 1; Myogenic factor 3 (Myf-3); Myogenin D1; PUM) (HRP); bHLHc1; PUM; anti-MyoD1 antibody

• Host: Mouse
• Reactivity: Chicken, Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 5.8A
• Specificity: Recognizes human MyoD1. Species Crossreactivity: mouse, rat, and chicken. Others-not known.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-MyoD1 antibody: Immunohistochemistry (IHC) Frozen/Paraffin, Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: WB: 0.25-0.5ug/ml; IHC-F/P: 0.5-1ug/ml for 30 minutes at RT; IP: 0.5-1ug/500ug protein lysate; Applications are based on unconjugated antibody.
• Immunogen: Recombinant mouse MyoD1 protein. Cellular Localization: Nuclear
• Conjugate: HRP
• Positive Control: Rhabdomyosarcoma
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with MyoD1 MAb (5.8A))

Product Categories/Family for anti-MyoD1 antibody

Antibodies; Transcription Factors

References

1. Tallini G; Parham DM; Dias P; Cordon-Cardo C; Houghton PJ; Rosai J. Myogenic regulatory protein expression in adult soft tissue sarcomas. A sensitive and specific marker of skeletal muscle differentiation. American Journal of Pathology, 1994, 144(4):693-701. 2. Dias P; Parham DM; Shapiro DN; Tapscott SJ; Houghton PJ. Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility. Cancer Research, 1992 Dec 1, 52(23):6431-9. 3. Rosai J; Dias P; Parham DM; Shapiro DN; Houghton P. MyoD1 protein expression in alveolar soft part sarcoma as confirmatory evidence of its skeletal muscle nature. Am Journal of Surgical Pathology, 1991, 15(10):974-81.

NCBI and Uniprot Product Information

• NCBI GI #: 23111009
• NCBI GeneID: 4654
• NCBI Accession #: NP_002469.2
• NCBI GenBank Nucleotide #: NM_002478.4
• UniProt Accession #: P15172; P10085
• Molecular Weight: 34,501 Da
• NCBI Official Full Name: myoblast determination protein 1
• NCBI Official Synonym Full Names: myogenic differentiation 1
• NCBI Official Symbol: MYOD1
• NCBI Official Synonym Symbols: PUM; MYF3; MYOD; bHLHc1
• NCBI Protein Information: myoblast determination protein 1; myf-3; myogenic factor 3; class C basic helix-loop-helix protein 1
• UniProt Protein Name: Myoblast determination protein 1
• Protein Family: Myoblast determination protein
• UniProt Gene Name: MYOD1
• UniProt Synonym Gene Names: BHLHC1; MYF3; MYOD; bHLHc1; Myf-3
• UniProt Entry Name: MYOD1_HUMAN

NCBI Description

This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

MyoD: a transcription factor of the basic helix-loop-helix family and the myogenic factors subfamily. Involved in muscle differentiation. Induces fibroblasts to differentiate into myoblasts. It is involved in muscle cell differentiation, and is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.

Protein type: DNA-binding; RNA splicing; Transcription factor

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: nucleoplasm; transcription factor complex; myofibril; nuclear chromatin; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; chromatin DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; transcription coactivator activity; transcription factor binding; nuclear hormone receptor binding

Biological Process: transcription from RNA polymerase II promoter; muscle development; skeletal muscle development; skeletal muscle fiber adaptation; positive regulation of skeletal muscle regeneration; regulation of RNA splicing; myotube differentiation involved in skeletal muscle regeneration; muscle cell fate commitment; skeletal muscle fiber development; myotube cell development; cellular response to starvation; protein amino acid phosphorylation; positive regulation of myoblast differentiation; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; positive regulation of skeletal muscle fiber development; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; myoblast cell fate determination; regulation of alternative nuclear mRNA splicing, via spliceosome