Western Blot (WB)
(Figure 1: Western blot analysis using MYL3 (1) and MYL2 (2) mouse mAb against rat fetal heart tissue lysate.)
Mouse anti-Human MYL2 Monoclonal Antibody | anti-MYL2 antibody
MYL2 Antibody
Gene Names
MYL9; LC20; MLC2; MRLC1; MYRL2; MLC-2C
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
Affinity-Chromatography
Synonyms
MYL2; Monoclonal Antibody; MYL2 Antibody; Myosin light chain regulatory B; Myosin regulatory light chain 2 B; smooth muscle isoform; RLC B; anti-MYL2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
MYL2 antibody detects endogenous levels of total MYL2
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
172
Applicable Applications for anti-MYL2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
IF/ICC: 1:100-1:500
WB: 1:500-1:2000
IF/ICC: 1:100-1:500
Immunogen
Purified recombinant fragment of human MYL2 expressed in E. Coli
Tissue Specificity
Smooth muscle tissues and in some, but not all, nonmuscle cells.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using MYL3 (1) and MYL2 (2) mouse mAb against rat fetal heart tissue lysate.)
Western Blot (WB)
(Figure 1: Western blot analysis using MYL3 (1) and MYL2 (2) mouse mAb against rat fetal heart tissue lysate.)
Related Product Information for anti-MYL2 antibody
Description: MYL2(myosin, light chain 2, regulatory, cardiac, slow), also known as MLC-2, MLC2v. Entrez protein NP_000423. MYL2 associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. It is an hexamer of two heavy chains and four light chains that is predominantly expressed in adult cardiac ventricle muscle.
Function: Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
Subunit Structure: Myosin is a hexamer of 2 heavy chains and 4 light chains: interacts with myosin heavy chain MYO19.
Post-translational Modifications: Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
Function: Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
Subunit Structure: Myosin is a hexamer of 2 heavy chains and 4 light chains: interacts with myosin heavy chain MYO19.
Post-translational Modifications: Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 19 kDa
Predicted: 20 kDa
Predicted: 20 kDa
NCBI Official Full Name
myosin regulatory light polypeptide 9 isoform a
NCBI Official Synonym Full Names
myosin light chain 9
NCBI Official Symbol
MYL9
NCBI Official Synonym Symbols
LC20; MLC2; MRLC1; MYRL2; MLC-2C
NCBI Protein Information
myosin regulatory light polypeptide 9
UniProt Protein Name
Myosin regulatory light polypeptide 9
Protein Family
UniProt Gene Name
MYL9
UniProt Synonym Gene Names
MLC2; MRLC1; MYRL2; LC20
NCBI Description
Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
Research Articles on MYL2
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Product Notes
The MYL2 myl9 (Catalog #AAA9602378) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MYL2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MYL2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). ELISA: 1:10000 WB: 1:500-1:2000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the MYL2 myl9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYL2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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