Mouse Myc Monoclonal Antibody | anti-Myc antibody

Myc-tag (10D6) Monoclonal Antibody

Cat. Num. AAA3014385

• Gene Names: MYC; MRTL; MYCC; c-Myc; bHLHe39
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Myc; Monoclonal Antibody; Myc-tag (10D6) Monoclonal Antibody; Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64; MYC; BHLHE39; anti-Myc antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Specificity: Myc-tag mAb detects endogenous levels of Myc-tag protein.
• Purity/Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 454

• Applicable Applications for anti-Myc antibody: Western Blot (WB)
• Application Notes: WB: 1:2000-1:10,000
• Immunogen: Synthetic peptide, corresponding Human Myc.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Myc-tag (10D6) mAb at 1:5000 dilutionLane1:AML-12 whole cell lysate (10ug) Lane2:PC12 whole cell lysate (10ug) Lane3:K562 whole cell lysate (10ug) Lane4:L02 whole cell lysate (10ug))

Related Product Information for anti-Myc antibody

Members of the Myc/Max/Mad network function as transcriptional regulators with roles in various aspects of cell behavior including proliferation, differentiation and apoptosis. These proteins share a common basic-helix-loop-helix leucine zipper (bHLH-ZIP) motif required for dimerization and DNA-binding. Max was originally discovered based on its ability to associate with c-Myc and found to be required for the ability of Myc to bind DNA and activate transcription. Subsequently, Max has been viewed as a central component of the transcriptional network, forming homodimers as well as heterodimers with other members of the Myc and Mad families. The association between Max and either Myc or Mad can have opposing effects on transcriptional regulation and cell behavior. The Mad family consists of four related proteins; Mad1, Mad2 (Mxi1), Mad3 and Mad4, and the more distantly related members of the bHLH-ZIP family, Mnt and Mga. Like Myc, the Mad proteins are tightly regulated with short half-lives. In general, Mad family members interfere with Myc-mediated processes such as proliferation, transformation and prevention of apoptosis by inhibiting transcription.

NCBI and Uniprot Product Information

• NCBI GI #: 71774083
• NCBI GeneID: 4609
• NCBI Accession #: NP_002458.2
• NCBI GenBank Nucleotide #: NP_002458.2
• UniProt Accession #: P01106
• Molecular Weight: ~ 49, 51kDa
• NCBI Official Full Name: myc proto-oncogene protein isoform 1
• NCBI Official Synonym Full Names: MYC proto-oncogene, bHLH transcription factor
• NCBI Official Symbol: MYC
• NCBI Official Synonym Symbols: MRTL; MYCC; c-Myc; bHLHe39
• NCBI Protein Information: myc proto-oncogene protein
• UniProt Protein Name: Myc proto-oncogene protein
• Protein Family: Mycosin
• UniProt Gene Name: MYC
• UniProt Synonym Gene Names: BHLHE39; bHLHe39
• UniProt Entry Name: MYC_HUMAN

NCBI Description

This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]

Uniprot Description

Myc: a proto-oncogenic transcription factor that plays a role in cell proliferation, apoptosis and in the development of human tumors. Seems to activate the transcription of growth-related genes.

Protein type: DNA-binding; Transcription factor; Oncoprotein; Nucleolus

Chromosomal Location of Human Ortholog: 8q24.21

Cellular Component: nucleoplasm; protein complex; nucleolus; nucleus; cytosol

Molecular Function: protein dimerization activity; protein binding; DNA binding; protein complex binding; transcription factor activity; transcription factor binding

Biological Process: oxygen transport; cellular iron ion homeostasis; positive regulation of transcription, DNA-dependent; positive regulation of caspase activity; Wnt receptor signaling pathway through beta-catenin; negative regulation of transcription from RNA polymerase II promoter; chromosome organization and biogenesis; positive regulation of fibroblast proliferation; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; response to gamma radiation; cell cycle arrest; response to drug; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; transcription, DNA-dependent; regulation of telomere maintenance; MAPKKK cascade; negative regulation of cell division; negative regulation of stress-activated MAPK cascade; negative regulation of monocyte differentiation; chromatin remodeling; ureteric bud branching; negative regulation of fibroblast proliferation; regulation of gene expression; energy reserve metabolic process; positive regulation of transcription from RNA polymerase II promoter; gene expression; response to DNA damage stimulus; positive regulation of epithelial cell proliferation; negative regulation of apoptosis

Disease: Burkitt Lymphoma