Mouse anti-Human MXI1 Monoclonal Antibody | anti-MXI1 antibody

Mouse monoclonal antibody Anti-Human MXI1

Cat. Num. AAA120419

• Gene Names: MXI1; MXI; MAD2; MXD2; bHLHc11
• Reactivity: Human
• Applications: Dot Blot
• Synonyms: MXI1; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human MXI1; Homo sapiens MAX interactor 1; mRNA (cDNA clone MGC:9999 IMAGE:3882557); complete cds.; MAD2; MXD2; MXI; MGC43220; anti-MXI1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: MXI1C2a
• Concentration: 100 ug/ml (1.0 ml) (varies by lot)
• Sequence Length: 2187

• Applicable Applications for anti-MXI1 antibody: Dot Blot (DB)
• Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Sterility: Filtered through a 0.22 um membrane.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-MXI1 monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Related Product Information for anti-MXI1 antibody

Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The protein encoded by this gene is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors. Three alternatively spliced transcripts encoding different isoforms have been described. Additional alternatively spliced transcripts may exist but the products of these transcripts have not been verified experimentally.[NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

• NCBI GI #: 15277707
• NCBI GeneID: 4601
• Molecular Weight: 20,558 Da
• NCBI Official Full Name: Homo sapiens MAX interactor 1, mRNA
• NCBI Official Synonym Full Names: MAX interactor 1, dimerization protein
• NCBI Official Symbol: MXI1
• NCBI Official Synonym Symbols: MXI; MAD2; MXD2; bHLHc11
• NCBI Protein Information: max-interacting protein 1; MAX dimerization protein 2; Max-related transcription factor; class C basic helix-loop-helix protein 11
• UniProt Protein Name: Max-interacting protein 1
• Protein Family: Max-interacting protein
• UniProt Gene Name: MXI1
• UniProt Entry Name: MXI1_HUMAN

NCBI Description

Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The protein encoded by this gene is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors. Three alternatively spliced transcripts encoding different isoforms have been described. Additional alternatively spliced transcripts may exist but the products of these transcripts have not been verified experimentally. [provided by RefSeq, Jul 2008]

Uniprot Description

Mad2: Transcriptional repressor. MXI1 binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MXI1 thus antagonizes MYC transcriptional activity by competing for MAX. Defects in MXI1 may be a cause of susceptibility to prostate cancer (PC). It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; DNA-binding

Chromosomal Location of Human Ortholog: 10q24-q25

Cellular Component: cytoplasm; nucleolus; nucleus

Molecular Function: protein dimerization activity; DNA binding; transcription corepressor activity

Biological Process: negative regulation of cell proliferation; cytoplasmic sequestering of transcription factor; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter

Disease: Prostate Cancer