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Western Blot (WB) (Western Blot detection against Immunogen (33.7kD).)

Mouse anti-Human MURF3 Monoclonal Antibody | anti-MURF3 antibody

MURF3 (Tripartite Motif-containing Protein 54, Muscle-specific RING Finger Protein, MuRF, Muscle-specific RING Finger Protein 3, MuRF-3, MuRF3, RING Finger Protein 30, MURF, TRIM54, RNF30) (AP)

Gene Names
TRIM54; MURF; RNF30; muRF3; MURF-3
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
MURF3; Monoclonal Antibody; MURF3 (Tripartite Motif-containing Protein 54; Muscle-specific RING Finger Protein; MuRF; Muscle-specific RING Finger Protein 3; MuRF-3; MuRF3; RING Finger Protein 30; MURF; TRIM54; RNF30) (AP); anti-MURF3 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
2G9
Specificity
Recognizes human TRIM54.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-MURF3 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa186-255 from human TRIM54 (NP_115935) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
PILASQNTKIIDSELSDGIAMLVAGNDRVQAVITQMEEVCQTIEDNSRRQKQLLNQRFESLCAVLEERK
Conjugate
AP
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (33.7kD).)

Western Blot (WB) (Western Blot detection against Immunogen (33.7kD).)
Related Product Information for anti-MURF3 antibody
May bind and stabilize microtubules during myotubes formation.
Product Categories/Family for anti-MURF3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
45kDa
NCBI Official Full Name
tripartite motif-containing protein 54 isoform 1
NCBI Official Synonym Full Names
tripartite motif containing 54
NCBI Official Symbol
TRIM54
NCBI Official Synonym Symbols
MURF; RNF30; muRF3; MURF-3
NCBI Protein Information
tripartite motif-containing protein 54
UniProt Protein Name
Tripartite motif-containing protein 54
UniProt Gene Name
TRIM54
UniProt Synonym Gene Names
MURF; MURF3; RNF30; MuRF; MuRF-3; MuRF3
UniProt Entry Name
TRI54_HUMAN

NCBI Description

The protein encoded by this gene contains a RING finger motif and is highly similar to the ring finger proteins RNF28/MURF1 and RNF29/MURF2. In vitro studies demonstrated that this protein, RNF28, and RNF29 form heterodimers, which may be important for the regulation of titin kinase and microtubule-dependent signal pathways in striated muscles. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

TRIM54: May bind and stabilize microtubules during myotubes formation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Ligase; EC 6.3.2.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: microtubule; microtubule associated complex; Z disc

Molecular Function: signal transducer activity; zinc ion binding; microtubule binding

Biological Process: negative regulation of microtubule depolymerization; multicellular organismal development; signal transduction; cell differentiation; microtubule-based process

Research Articles on MURF3

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Product Notes

The MURF3 trim54 (Catalog #AAA6132431) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MURF3 (Tripartite Motif-containing Protein 54, Muscle-specific RING Finger Protein, MuRF, Muscle-specific RING Finger Protein 3, MuRF-3, MuRF3, RING Finger Protein 30, MURF, TRIM54, RNF30) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MURF3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MURF3 trim54 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MURF3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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