Mouse anti-Human. MUC18 Monoclonal Antibody | anti-MCAM antibody
MUC18/ CD146
Gene Names
MCAM; CD146; MUC18
Reactivity
Human.
Applications
ELISA, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Immunohistochemistry
Synonyms
MUC18; Monoclonal Antibody; MUC18/ CD146; Cell Surface Glycoprotein MUC18; Cell Surface Glycoprotein P1H12; Gicerin; MCAM; Melanoma Adhesion Molecule; Melanoma Associated Glycoprotein MUC18; Melanoma Cell Adhesion Molecule; Melanoma-associated Antigen A32; Mel-CAM; S-endo 1 Endothelial-associated Antigen; Sendo1; anti-MCAM antibody
Host
Mouse
Reactivity
Human.
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
OJ79c
Specificity
The human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
527
Applicable Applications for anti-MCAM antibody
ELISA (For coating, order antibody without BSA), Flow Cytometry (0.5-1ug/million cells), Immunofluorescence (0.5-1.0ug/ml), Immunoprecipitation (1-2ug/500ug protein), Western Blot (0.5-1.0ug/ml), Immunohistology (Frozen) (0.5-1.0ug/ml for 30 min at RT Opt
Application Notes
ELISA (For coating, order antibody without BSA); Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1.0ug/ml); Immunoprecipitation (1-2ug/500ug protein); Western Blotting (0.5-1.0ug/ml); Immunohistology (Frozen) (0.5-1.0ug/ml for 30 min at RT Optimal dilution for a specific application should be determined.
Localization
Cell surface
Positive Control
A-375, HUV-EC or HeLa Cells. Tonsil or Melanoma.
Human Chromosomal Location
11q23.3
Immunogen
Recombinant human MUC18 protein
Quantity and Buffer
0.5 ml at 200ug/ml with BSA and azide
Preparation and Storage
Store at 2 to 8 degree C. Antibody is stable for 24 months.
References
1. Pruszak J et al. Stem Cells 25:2257-68 (2007)
NCBI and Uniprot Product Information
NCBI GeneID
Molecular Weight
130kDa
NCBI Official Full Name
cell surface glycoprotein MUC18
NCBI Official Synonym Full Names
melanoma cell adhesion molecule
NCBI Official Symbol
MCAM
NCBI Official Synonym Symbols
CD146; MUC18
NCBI Protein Information
cell surface glycoprotein MUC18
UniProt Protein Name
Cell surface glycoprotein MUC18
Protein Family
UniProt Gene Name
MCAM
UniProt Synonym Gene Names
MUC18
UniProt Entry Name
MUC18_HUMAN
Uniprot Description
MCAM: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q23.3
Cellular Component: extracellular space; focal adhesion; integral to membrane; plasma membrane; nucleus; external side of plasma membrane
Biological Process: anatomical structure morphogenesis; glomerular filtration; angiogenesis; cell adhesion; positive regulation of cell migration
Research Articles on MCAM
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Product Notes
The MCAM mcam (Catalog #AAA438396) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MUC18/ CD146 reacts with Human. and may cross-react with other species as described in the data sheet. AAA Biotech's MUC18 can be used in a range of immunoassay formats including, but not limited to, ELISA (For coating, order antibody without BSA), Flow Cytometry (0.5-1ug/million cells), Immunofluorescence (0.5-1.0ug/ml), Immunoprecipitation (1-2ug/500ug protein), Western Blot (0.5-1.0ug/ml), Immunohistology (Frozen) (0.5-1.0ug/ml for 30 min at RT Opt. ELISA (For coating, order antibody without BSA); Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1.0ug/ml); Immunoprecipitation (1-2ug/500ug protein); Western Blotting (0.5-1.0ug/ml); Immunohistology (Frozen) (0.5-1.0ug/ml for 30 min at RT Optimal dilution for a specific application should be determined. Researchers should empirically determine the suitability of the MCAM mcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MUC18, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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