Mouse anti-Human MSH6 Monoclonal Antibody | anti-MSH6 antibody

MSH6 Monoclonal Antibody

Cat. Num. AAA3014327

• Gene Names: MSH6; GTBP; HSAP; p160; GTMBP; HNPCC5
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry
• Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MSH6; Monoclonal Antibody; MSH6 Monoclonal Antibody; DNA mismatch repair protein Msh6; hMSH6; G/T mismatch-binding protein; GTBP; GTMBP; MutS-alpha 160 kDa subunit; p160; anti-MSH6 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Specificity: This antibody detects endogenous levels of MSH6 and does not cross-react with related proteins
• Purity/Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1360

• Applicable Applications for anti-MSH6 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB: 1:300-500; ICC: 1:50-100
• Immunogen: Recombinant full length Human MSH6.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot detection of MSH6 antibody in 293 and HCT116 cell lysates using MSH6 antibody (1:300-1:500 diluted).)

Immunocytochemistry (ICC)

(Immunocytochemistry staining of HeLa cells using anti-MSH6 antibody (dilution 1:100).)

Related Product Information for anti-MSH6 antibody

Component of the post-replicative DNA mismatch repair system (MMR).Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair.When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA.After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.ATP binding and hydrolysis play a pivotal role in mismatch repair functions.The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone.This transition is crucial for mismatch repair.MutS alpha may also play a role in DNA homologous recombination repair.

NCBI and Uniprot Product Information

• NCBI GI #: 4504191
• NCBI GeneID: 2956
• NCBI Accession #: NP_000170.1
• NCBI GenBank Nucleotide #: NP_000170.1
• UniProt Accession #: P52701
• Molecular Weight: Predicted Band Size: 160KDa; Observed Band Size: 160KDa
• NCBI Official Full Name: DNA mismatch repair protein Msh6 isoform 1
• NCBI Official Synonym Full Names: mutS homolog 6
• NCBI Official Symbol: MSH6
• NCBI Official Synonym Symbols: GTBP; HSAP; p160; GTMBP; HNPCC5
• NCBI Protein Information: DNA mismatch repair protein Msh6
• UniProt Protein Name: DNA mismatch repair protein Msh6
• Protein Family: DNA mismatch repair protein
• UniProt Gene Name: MSH6
• UniProt Synonym Gene Names: GTBP; hMSH6; GTBP; GTMBP; p160
• UniProt Entry Name: MSH6_HUMAN

NCBI Description

This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. [provided by RefSeq, Jul 2013]