Mouse (C3H/HeJ) Anti-Mouse H-2Kd/H-2Dd Secondary Antibody
Mouse Anti-Mouse H-2Kd/H-2Dd-UNLB
Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry
Synonyms
Mouse (C3H/HeJ) Anti-Mouse H-2Kd/H-2Dd; Monoclonal Secondary Antibody; Mouse Anti-Mouse H-2Kd/H-2Dd-UNLB; Mouse Anti-Mouse H-2Kd/H-2Dd - Purified; Unlabeled Antibody; Mouse (C3H/HeJ) Anti-Mouse H-2Kd/H-2Dd secondary antibody
Host
Mouse
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG2a kappa
Clone Number
34-1-2S
Specificity
An epitope in the alpha 3 domain that is common to H-2Kd and H-2Dd
The "classical" MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta 2-microglobulin.3,4 These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells.5 MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.3,6 The monoclonal antibody 34-1-2S binds to a common determinant in the alpha 3 domains of H-2Kd and H-2Dd in the presence or absence of beta 2 microglobulin.7,8 It cross reacts with the alpha 3 domain of H-2Kb.1,2
The "classical" MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta 2-microglobulin.3,4 These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells.5 MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.3,6 The monoclonal antibody 34-1-2S binds to a common determinant in the alpha 3 domains of H-2Kd and H-2Dd in the presence or absence of beta 2 microglobulin.7,8 It cross reacts with the alpha 3 domain of H-2Kb.1,2
Form/Format
Purified (UNLB) Antibody
Applicable Applications for Mouse (C3H/HeJ) Anti-Mouse H-2Kd/H-2Dd secondary antibody
Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections)
Application Notes
Research Application(s): Complement-mediated cytotoxicity
Flow Cytometry Suggested Dilution: <=1 ug/10^6 cells
Flow Cytometry Suggested Dilution: <=1 ug/10^6 cells
Immunogen
C3H alpha BDF1 mouse splenocytes
Characterization
To ensure lot to lot consistency, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Ozato, K., T.H. Hansen, and D.H. Sachs. 1980. J. Immunol. 125:2473.
2. Ozato, K., and D.H. Sachs. 1981. J. Immunol. 126:317.
3. Lawlor, D.A., et al. 1990. Annu. Rev. Immunol. 8:23.
4. Bjorkman, P.J., et al. 1987. Nature 329:506.
5. Yewdell, J.W., and J.R. Bennett. 1990. Cell 62:203.
6. Zijlstra, M., et al. 1990. Nature 344:742.
7. Allen, H., D. Wraith, P. Pala, B. Askonas, and R.A. Flavell. 1984. Nature 309:279.
8. Allen, H, J. Fraser, D. Flyer, S. Calvin, and R. Flavell. 1986. Proc. Natl. Acad. Sci. USA 83:7447
2. Ozato, K., and D.H. Sachs. 1981. J. Immunol. 126:317.
3. Lawlor, D.A., et al. 1990. Annu. Rev. Immunol. 8:23.
4. Bjorkman, P.J., et al. 1987. Nature 329:506.
5. Yewdell, J.W., and J.R. Bennett. 1990. Cell 62:203.
6. Zijlstra, M., et al. 1990. Nature 344:742.
7. Allen, H., D. Wraith, P. Pala, B. Askonas, and R.A. Flavell. 1984. Nature 309:279.
8. Allen, H, J. Fraser, D. Flyer, S. Calvin, and R. Flavell. 1986. Proc. Natl. Acad. Sci. USA 83:7447
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Product Notes
The Mouse (C3H/HeJ) Anti-Mouse H-2Kd/H-2Dd (Catalog #AAA673684) is a Secondary Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Mouse H-2Kd/H-2Dd-UNLB reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Mouse (C3H/HeJ) Anti-Mouse H-2Kd/H-2Dd can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections). Research Application(s): Complement-mediated cytotoxicity Flow Cytometry Suggested Dilution: <=1 ug/10^6 cells. Researchers should empirically determine the suitability of the Mouse (C3H/HeJ) Anti-Mouse H-2Kd/H-2Dd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mouse (C3H/HeJ) Anti-Mouse H-2Kd/H-2Dd, Monoclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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