Mouse Anti-Rabbit RLA-DQ Secondary Antibody
Mouse Anti-Rabbit RLA-DQ-FITC
Reactivity
Rabbit
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Synonyms
Mouse Anti-Rabbit RLA-DQ; Monoclonal Secondary Antibody; Mouse Anti-Rabbit RLA-DQ-FITC; Mouse Anti-Rabbit RLA-DQ - FITC; Mouse Anti-Rabbit RLA-DQ secondary antibody
Host
Mouse
Reactivity
Rabbit
Clonality
Monoclonal
Isotype
Mouse (BALB/c) IgG2a kappa
Clone Number
2C4
Specificity
Rabbit RLA-DQ alpha chain (Mr ~35 kDa)
MAb 2C4 reacts with RLA-DQ-transfected cells, but not with RLA-DR-transfected cells2. In normal adult rabbits, the antibody reacts with 30-60% of peripheral blood leukocytes.2,3 It has also been reported that the antibody stains: lymphocytes and macrophages in the spleen; lymphocytes in the thymic medulla and some cells in the cortex; Kupffer cells in the liver; and synovial lining cells (i.e., infiltrating macrophages) in inflamed synovium.4 DQ alpha transcripts have been detected in bone marrow and spleen and, to a lesser extent, in appendix, lymph nodes, and other organs.5
MAb 2C4 reacts with RLA-DQ-transfected cells, but not with RLA-DR-transfected cells2. In normal adult rabbits, the antibody reacts with 30-60% of peripheral blood leukocytes.2,3 It has also been reported that the antibody stains: lymphocytes and macrophages in the spleen; lymphocytes in the thymic medulla and some cells in the cortex; Kupffer cells in the liver; and synovial lining cells (i.e., infiltrating macrophages) in inflamed synovium.4 DQ alpha transcripts have been detected in bone marrow and spleen and, to a lesser extent, in appendix, lymph nodes, and other organs.5
Form/Format
Fluorescein (FITC) Conjugate
Applicable Applications for Mouse Anti-Rabbit RLA-DQ secondary antibody
Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation
Application Notes
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Immunogen
Rabbit spleen cells
Characterization
To ensure acceptable performance, each batch of product is tested by flow cytometry to conform to the characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
References
1. Lobel, S.A., and K.L. Knight. 1984. The role of rabbit Ia molecules in immune functions as determined with the use of an anti-Ia monoclonal antibody. Immunology 51:35-43.
2. Spieker-Polet, H., N. Sittisombut, P.-C. Yam, and K.L. Knight. 1990. Rabbit major histocompatibility complex IV. Expression of major histocompatibility complex class II genes. J. Immunogenetics 17:123-132.
4. Wilkinson, J. M., G. McDonald, S. Smith, J. Galea-Lauri, J. Lewthwaite, B. Henderson, and P.A. Revell. 1993. Immunohistochemical identification of leucocyte populations in normal tissue and inflamed synovium of the rabbit. J. Pathol. 170:315-320.
5. Kulaga, H., J.-A. Sogn, J.D. Weissman, P.N. Marche, C. LeGuern, E.O. Long, and T.J. Kindt. 1987. Expression patterns of MHC class II genes in rabbit tissues indicate close homology to human counterparts. J. Immunol. 139:587-592.
2. Spieker-Polet, H., N. Sittisombut, P.-C. Yam, and K.L. Knight. 1990. Rabbit major histocompatibility complex IV. Expression of major histocompatibility complex class II genes. J. Immunogenetics 17:123-132.
4. Wilkinson, J. M., G. McDonald, S. Smith, J. Galea-Lauri, J. Lewthwaite, B. Henderson, and P.A. Revell. 1993. Immunohistochemical identification of leucocyte populations in normal tissue and inflamed synovium of the rabbit. J. Pathol. 170:315-320.
5. Kulaga, H., J.-A. Sogn, J.D. Weissman, P.N. Marche, C. LeGuern, E.O. Long, and T.J. Kindt. 1987. Expression patterns of MHC class II genes in rabbit tissues indicate close homology to human counterparts. J. Immunol. 139:587-592.
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Product Notes
The Mouse Anti-Rabbit RLA-DQ (Catalog #AAA673788) is a Secondary Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Rabbit RLA-DQ-FITC reacts with Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's Mouse Anti-Rabbit RLA-DQ can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation. Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells. Researchers should empirically determine the suitability of the Mouse Anti-Rabbit RLA-DQ for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mouse Anti-Rabbit RLA-DQ, Monoclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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