Mouse Anti-Mouse H-2Dd Secondary Antibody
Mouse Anti-Mouse H-2Dd-FITC
Reactivity
Mouse
Applications
Flow Cytometry, Immunoprecipitation
Synonyms
Mouse Anti-Mouse H-2Dd; Monoclonal Secondary Antibody; Mouse Anti-Mouse H-2Dd-FITC; Mouse Anti-Mouse H-2Dd - FITC; Mouse Anti-Mouse H-2Dd secondary antibody
Host
Mouse
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Mouse (C3H alpha BDF1) IgG2a kappa
Clone Number
34-5-8S
Specificity
A conformational epitope on the alpha1 and alpha2 chains of mouse H-2Dd class I alloantigen
MAb 34-5-8S reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of alpha1 and alpha2 chains when complexed with beta 2-microglobulin.2 The antibody does not react with H-2Dd alpha chains synthesized in vitro.3 Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H- 2s haplotypes has been observed by flow cytometric analysis.4 Reactivity with cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes has not been observed.2 MAb 34-5-8S has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells.5-9
MAb 34-5-8S reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of alpha1 and alpha2 chains when complexed with beta 2-microglobulin.2 The antibody does not react with H-2Dd alpha chains synthesized in vitro.3 Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H- 2s haplotypes has been observed by flow cytometric analysis.4 Reactivity with cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes has not been observed.2 MAb 34-5-8S has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells.5-9
Form/Format
Fluorescein (FITC) Conjugate
Applicable Applications for Mouse Anti-Mouse H-2Dd secondary antibody
Flow Cytometry; Immunoprecipitation; Immunoaffinity Chromatography
Application Notes
Research Application(s): Complement-dependent cytotoxicity
Blocking of antigen recognition
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Blocking of antigen recognition
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Immunogen
C57BL/6 x DBA/2)F1 hybrid mouse splenocytes1
Characterization
To ensure acceptable performance, each batch of product is tested by flow cytometry to conform to the characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Ozato, K., N. Mayer, and D.H. Sachs. 1980. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J. Immunol. 124:533-540.
2. Evans, G.A., D.H. Margulies, B. Shykind, J.G. Seidman, and K. Ozato. 1982. Exon shuffling: Mapping polymorphic determinants of mouse transplantation antigens. Transplantation 34:113-120.
3. Otten, G.R., E. Bikoff, R.K. Ribaudo, S. Kozlowski, D.H. Margulies, and R.N. Germain. 1992. Peptide and b2-microglobulin regulation of cell surface MHC class I conformation and expression. J. Immunol. 148:3723-3732.
5. Kane. K.P. 1994. Ly-49 mediates EL4 lymphoma adhesion to isolated class I major histocompatibility complex molecules. J. Exp. Med. 179:1011-1015.
6. Daniels,.F., F.M. Karlhofer, W.E. Seamna, and W.M. Yokoyama. 1994. A natural killer cell receptor specific for a major histocompatibility class I molecule. J. Exp. Med. 180:687-692.
7. Mason, L.H., J.R. Ortaldo, H.A. Young, V. Kumar, M. Bennett, and S.K. Anderson. 1995. Cloning and functional characteristics of murine large granular lymphocyte-1: A member of the Ly-49 gene family (Ly-49G2). J. Exp. Med. 182:293-303.
8. Brea\nnan, J., G. Mahon, D.L. Mager, W.A. Jefferies, and F. Takei. 1996. Recognition of class I major histocompatible complex molecules by Ly-49: Specificities and domain interactions. J. Exp. Med. 183:1553-1559.
9. Orihuela, M., D.H. Margulies, and W.M. Yokoyama. 1996. The natural killer cell receptor Ly-49A recognizes a peptideinduced conformational determinant on its major histocompatible complex class I ligand. Proc. Natl. Acad. Sci. USA 93:11792-11797.
10. Beck, J.C., T.H. Hansen, S.E. Cullen, and D.R. Lee. 1986. Slower processing, weaker ?2-microglobulin association, and lower surface expression of H-2Ld are influenced by its amino terminus. J. Immunol. 137:916-923.
2. Evans, G.A., D.H. Margulies, B. Shykind, J.G. Seidman, and K. Ozato. 1982. Exon shuffling: Mapping polymorphic determinants of mouse transplantation antigens. Transplantation 34:113-120.
3. Otten, G.R., E. Bikoff, R.K. Ribaudo, S. Kozlowski, D.H. Margulies, and R.N. Germain. 1992. Peptide and b2-microglobulin regulation of cell surface MHC class I conformation and expression. J. Immunol. 148:3723-3732.
5. Kane. K.P. 1994. Ly-49 mediates EL4 lymphoma adhesion to isolated class I major histocompatibility complex molecules. J. Exp. Med. 179:1011-1015.
6. Daniels,.F., F.M. Karlhofer, W.E. Seamna, and W.M. Yokoyama. 1994. A natural killer cell receptor specific for a major histocompatibility class I molecule. J. Exp. Med. 180:687-692.
7. Mason, L.H., J.R. Ortaldo, H.A. Young, V. Kumar, M. Bennett, and S.K. Anderson. 1995. Cloning and functional characteristics of murine large granular lymphocyte-1: A member of the Ly-49 gene family (Ly-49G2). J. Exp. Med. 182:293-303.
8. Brea\nnan, J., G. Mahon, D.L. Mager, W.A. Jefferies, and F. Takei. 1996. Recognition of class I major histocompatible complex molecules by Ly-49: Specificities and domain interactions. J. Exp. Med. 183:1553-1559.
9. Orihuela, M., D.H. Margulies, and W.M. Yokoyama. 1996. The natural killer cell receptor Ly-49A recognizes a peptideinduced conformational determinant on its major histocompatible complex class I ligand. Proc. Natl. Acad. Sci. USA 93:11792-11797.
10. Beck, J.C., T.H. Hansen, S.E. Cullen, and D.R. Lee. 1986. Slower processing, weaker ?2-microglobulin association, and lower surface expression of H-2Ld are influenced by its amino terminus. J. Immunol. 137:916-923.
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Product Notes
The Mouse Anti-Mouse H-2Dd (Catalog #AAA673689) is a Secondary Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Mouse H-2Dd-FITC reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Mouse Anti-Mouse H-2Dd can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunoprecipitation; Immunoaffinity Chromatography. Research Application(s): Complement-dependent cytotoxicity Blocking of antigen recognition Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells. Researchers should empirically determine the suitability of the Mouse Anti-Mouse H-2Dd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mouse Anti-Mouse H-2Dd, Monoclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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