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Western Blot (WB) (Western BlotPositive WB detected in: HepG2 whole cell lysate, SH-SY5Y whole cell lysateAll lanes: Mono-methyl-Histone H3.1 (R128)antibody at 0.87ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 15 KDaObserved band size: 15 KDa)

Mono-methyl-Histone H3.1 Recombinant Antibody | HIST1H3A recombinant antibody

Mono-methyl-Histone H3.1 (R128) Antibody

Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Affinity-chromatography
Synonyms
Mono-methyl-Histone H3.1; Monoclonal Recombinant Antibody; Mono-methyl-Histone H3.1 (R128) Antibody; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; H3FA; AND; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; HIST1H3A recombinant antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity-chromatography
Form/Format
Liquid
Sequence Length
136
Applicable Applications for HIST1H3A recombinant antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide
Conjugation
Non-conjugated
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: HepG2 whole cell lysate, SH-SY5Y whole cell lysateAll lanes: Mono-methyl-Histone H3.1 (R128)antibody at 0.87ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 15 KDaObserved band size: 15 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: HepG2 whole cell lysate, SH-SY5Y whole cell lysateAll lanes: Mono-methyl-Histone H3.1 (R128)antibody at 0.87ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 15 KDaObserved band size: 15 KDa)
Product Categories/Family for HIST1H3A recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,404 Da
NCBI Official Full Name
histone H3.1
NCBI Official Synonym Full Names
histone cluster 1 H3 family member a
NCBI Official Symbol
HIST1H3A
NCBI Official Synonym Symbols
H3/A; H3FA
NCBI Protein Information
histone H3.1
UniProt Protein Name
Histone H3.1
UniProt Gene Name
HIST1H3A
UniProt Synonym Gene Names
H3FA

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Uniprot Description

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Research Articles on HIST1H3A

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Product Notes

The HIST1H3A hist1h3a (Catalog #AAA7110768) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Mono-methyl-Histone H3.1 (R128) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Mono-methyl-Histone H3.1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the HIST1H3A hist1h3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mono-methyl-Histone H3.1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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