Mouse MKNK2 Monoclonal Antibody | anti-MKNK2 antibody

MKNK2 (MAP Kinase Interacting Serine/Threonine Kinase 2, GPRK7, MNK2) (HRP)

Cat. Num. AAA6179901

• Gene Names: MKNK2; MNK2; GPRK7
• Applications: Western Blot
• Purity: Purified
• Synonyms: MKNK2; Monoclonal Antibody; MKNK2 (MAP Kinase Interacting Serine/Threonine Kinase 2; GPRK7; MNK2) (HRP); MAP Kinase Interacting Serine/Threonine Kinase 2; MNK2; anti-MKNK2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2A10
• Specificity: Recognizes MKNK2.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
• Sequence Length: 158

• Applicable Applications for anti-MKNK2 antibody: Western Blot (WB), In situ Proximity Ligation Assay (Cell)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: MKNK2 (AAH18345, 1aa-158aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MAPEVVEAFSEEASIYDKRCDLWSLGVILYILLSGYPPFVGRCGSDCGWDRGEACPACQNMLFESIQEGKYEFPDKDWAHISCAAKDLISKLLVRDAKQRLSAAQVLQHPWVQGCAPENTLPTPMVLQRWDSHFLLPPHPCRIHVRPGGLVRTVTVNE
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MKNK2 monoclonal antibody (M07), clone 2A10 Western Blot analysis of MKNK2 expression in K-562 (Cat # L009V1).)

Testing Data

(Detection limit for recombinant GST tagged MKNK2 is approximately 0.3ng/ml as a capture antibody.)

Western Blot (WB)

(MKNK2 monoclonal antibody (M07), clone 2A10. Western Blot analysis of MKNK2 expression in NIH/3T3 (Cat # L018V1).)

Western Blot (WB)

(MKNK2 monoclonal antibody (M07), clone 2A10. Western Blot analysis of MKNK2 expression in PC-12 (Cat # L012V1).)

Western Blot (WB)

(Western Blot analysis of MKNK2 expression in transfected 293T cell line by MKNK2 monoclonal antibody (M07), clone 2A10.Lane 1: MKNK2 transfected lysate (46.7 KDa).Lane 2: Non-transfected lysate.)

Testing Data

(Proximity Ligation Analysis of protein-protein interactions between MAPK14 and MKNK2 HeLa cells were stained with anti-MAPK14 rabbit purified polyclonal 1:1200 and anti-MKNK2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Product Categories/Family for anti-MKNK2 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 17390807
• NCBI GeneID: 2872
• NCBI Official Full Name: MKNK2 protein
• NCBI Official Synonym Full Names: MAPK interacting serine/threonine kinase 2
• NCBI Official Symbol: MKNK2
• NCBI Official Synonym Symbols: MNK2; GPRK7
• NCBI Protein Information: MAP kinase-interacting serine/threonine-protein kinase 2
• Protein Family: MAP kinase-interacting serine/threonine-protein kinase

NCBI Description

This gene encodes a member of the calcium/calmodulin-dependent protein kinases (CAMK) Ser/Thr protein kinase family, which belongs to the protein kinase superfamily. This protein contains conserved DLG (asp-leu-gly) and ENIL (glu-asn-ile-leu) motifs, and an N-terminal polybasic region which binds importin A and the translation factor scaffold protein eukaryotic initiation factor 4G (eIF4G). This protein is one of the downstream kinases activated by mitogen-activated protein (MAP) kinases. It phosphorylates the eukaryotic initiation factor 4E (eIF4E), thus playing important roles in the initiation of mRNA translation, oncogenic transformation and malignant cell proliferation. In addition to eIF4E, this protein also interacts with von Hippel-Lindau tumor suppressor (VHL), ring-box 1 (Rbx1) and Cullin2 (Cul2), which are all components of the CBC(VHL) ubiquitin ligase E3 complex. Multiple alternatively spliced transcript variants have been found, but the full-length nature and biological activity of only two variants are determined. These two variants encode distinct isoforms which differ in activity and regulation, and in subcellular localization. [provided by RefSeq, Aug 2011]