Mouse anti-Human MID1 Monoclonal Antibody | anti-MID1 antibody

MID1 (Midline-1, Midin, Midline 1 RING Finger Protein, Putative Transcription Factor XPRF, RING Finger Protein 59, Tripartite Motif-containing Protein 18, FXY, RNF59, TRIM18, XPRF) APC

Cat. Num. AAA6137624

• Gene Names: MID1; OS; FXY; OSX; OGS1; XPRF; BBBG1; GBBB1; MIDIN; RNF59; ZNFXY; TRIM18
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MID1; Monoclonal Antibody; MID1 (Midline-1; Midin; Midline 1 RING Finger Protein; Putative Transcription Factor XPRF; RING Finger Protein 59; Tripartite Motif-containing Protein 18; FXY; RNF59; TRIM18; XPRF) APC; anti-MID1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 2C11
• Specificity: Recognizes human MID1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-MID1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa441-540 from human MID1 (AAH53626) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: PNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGR
• Conjugate: APC
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (36.63kD).)

Related Product Information for anti-MID1 antibody

May have E3 ubiquitin ligase activity which targets the catalytic subunit of protein phosphatase 2 for degradation.

Product Categories/Family for anti-MID1 antibody

Antibodies; Enzymes

References

1. Control of mTORC1 signaling by the Opitz syndrome protein MID1. Liu E, Knutzen CA, Krauss S, Schweiger S, Chiang GG.Proc Natl Acad Sci U S A. 2011 May 24;108(21):8680-5. Epub 2011 May 9.

NCBI and Uniprot Product Information

• NCBI GI #: 31565477
• NCBI GeneID: 4281
• Molecular Weight: 62,288 Da
• NCBI Official Full Name: Homo sapiens midline 1 (Opitz/BBB syndrome), mRNA
• NCBI Official Synonym Full Names: midline 1
• NCBI Official Symbol: MID1
• NCBI Official Synonym Symbols: OS; FXY; OSX; OGS1; XPRF; BBBG1; GBBB1; MIDIN; RNF59; ZNFXY; TRIM18
• NCBI Protein Information: E3 ubiquitin-protein ligase Midline-1
• Protein Family: Mid1-interacting protein

NCBI Description

The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Multiple different transcript variants are generated by alternate splicing; however, the full-length nature of some of the variants has not been determined. [provided by RefSeq, Jul 2010]