Mouse anti-Mouse, Rat MHC Class 2, I-A Monoclonal Antibody | anti-orla-uaa antibody
MHC Class 2, I-A
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
MHC Class 2; I-A; Monoclonal Antibody; Anti -MHC Class 2; anti-orla-uaa antibody
Host
Mouse
Reactivity
Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3H440
Specificity
Recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Crossreacts with certain mouse strains of MHC haplotypes k and s and analysis of recombinant strains showed that the determinants mapped to the I-A region of the mouse MHC. Species Crossreactivity: Mouse. Does not recognize BDIX rat strain.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-orla-uaa antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot.
Dilution: Flow Cytometry: 1:50-1:100: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Immunohistochemistry (Frozen and paraffin sections): 1:50-1:100
Note: This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde)-refer to Whiteland et al. This clone is suitable for use on paraffin embedded material using target unmasking fluid-refer to McKechnie, N.M. et al., and has also been reported for application in Western Blot by Burrows, G.G. et al. (1998). This product does not require antigen retrieval using heat treatment or protein digestion prior to staining but results may be enhanced with heat treatment using 0.0M citrate buffer pH 6.0.
Optimal working dilutions to be determined by researcher.
Dilution: Flow Cytometry: 1:50-1:100: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Immunohistochemistry (Frozen and paraffin sections): 1:50-1:100
Note: This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde)-refer to Whiteland et al. This clone is suitable for use on paraffin embedded material using target unmasking fluid-refer to McKechnie, N.M. et al., and has also been reported for application in Western Blot by Burrows, G.G. et al. (1998). This product does not require antigen retrieval using heat treatment or protein digestion prior to staining but results may be enhanced with heat treatment using 0.0M citrate buffer pH 6.0.
Optimal working dilutions to be determined by researcher.
Immunogen
Rat Thymocyte membrane glycoprotein.
Recommended Negative Controls
For rat tissues only
I1904-78Y: IgG1 Murine Negative Control.
I1904-78Y: IgG1 Murine Negative Control.
Hybridoma
NS1 myeloma cells with spleen cells from Balb/c mice.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Product Categories/Family for anti-orla-uaa antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Mhc class I A
NCBI Official Symbol
orla-uaa
NCBI Protein Information
Mhc class I A
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Product Notes
The orla-uaa (Catalog #AAA631931) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MHC Class 2, I-A reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MHC Class 2, I-A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Dilution: Flow Cytometry: 1:50-1:100: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Immunohistochemistry (Frozen and paraffin sections): 1:50-1:100 Note: This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde)-refer to Whiteland et al. This clone is suitable for use on paraffin embedded material using target unmasking fluid-refer to McKechnie, N.M. et al., and has also been reported for application in Western Blot by Burrows, G.G. et al. (1998). This product does not require antigen retrieval using heat treatment or protein digestion prior to staining but results may be enhanced with heat treatment using 0.0M citrate buffer pH 6.0. Optimal working dilutions to be determined by researcher. Researchers should empirically determine the suitability of the orla-uaa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MHC Class 2, I-A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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