Flow Cytometry (FC/FACS)
(Figure-1: Cell surface flowcytometric staining of mFR4 (Clone: TH6) in mouse Thymocytes. Mouse Thymocytes were first stained with Anti-mCD3 APC conjugated, mCD3 APC positive cells were gated and further analyzed for mFR4 using 0.5 ug/10^6. Goat anti-Rat PE secondary antibody was used.)
Rat anti-Mouse mFolate Receptor 4 Monoclonal Antibody | anti-Izumo1r antibody
Monoclonal Antibody to mFolate Receptor 4 (Clone: TH6)
Gene Names
Izumo1r; Juno; Folr4; C86255; Folbp3; 0910001L11Rik
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
PurifiedProtein G Chromatography
Synonyms
mFolate Receptor 4; Monoclonal Antibody; Monoclonal Antibody to mFolate Receptor 4 (Clone: TH6); Sperm-egg fusion protein Juno; Folate receptor delta; Folate-binding protein 3; IZUMO1 receptor protein JUNO; FR4; anti-Izumo1r antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
TH6
Purity/Purification
Purified
Protein G Chromatography
Protein G Chromatography
Form/Format
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Sequence Length
244
Applicable Applications for anti-Izumo1r antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: 0.5-1 ug/10^6
Antigen
Folate Receptor 4
Immunogen Information
Tissue/Cell of mouse Spleen and Lymph node were used as the immunogen for this antibody.
Conjugation
Unconjugated
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeat freezing and thawing
For long-term storage, store at -20 degree C.
Avoid repeat freezing and thawing
Flow Cytometry (FC/FACS)
(Figure-1: Cell surface flowcytometric staining of mFR4 (Clone: TH6) in mouse Thymocytes. Mouse Thymocytes were first stained with Anti-mCD3 APC conjugated, mCD3 APC positive cells were gated and further analyzed for mFR4 using 0.5 ug/10^6. Goat anti-Rat PE secondary antibody was used.)
Flow Cytometry (FC/FACS)
(Figure-1: Cell surface flowcytometric staining of mFR4 (Clone: TH6) in mouse Thymocytes. Mouse Thymocytes were first stained with Anti-mCD3 APC conjugated, mCD3 APC positive cells were gated and further analyzed for mFR4 using 0.5 ug/10^6. Goat anti-Rat PE secondary antibody was used.)
Flow Cytometry (FC/FACS)
(Figure-2: Cell surface flowcytometric staining of mFR4 (Clone: TH6) in mouse Splenocytes. Mouse Splenocytes were first stained with Anti-mCD3 APC conjugated, mCD3 APC positive cells were gated and further analyzed for mFR4 using 0.5 ug/10^6. Goat anti-Rat PE secondary antibody was used.)
Flow Cytometry (FC/FACS)
(Figure-2: Cell surface flowcytometric staining of mFR4 (Clone: TH6) in mouse Splenocytes. Mouse Splenocytes were first stained with Anti-mCD3 APC conjugated, mCD3 APC positive cells were gated and further analyzed for mFR4 using 0.5 ug/10^6. Goat anti-Rat PE secondary antibody was used.)
Product Categories/Family for anti-Izumo1r antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,131 Da
NCBI Official Full Name
sperm-egg fusion protein Juno isoform 1
NCBI Official Synonym Full Names
IZUMO1 receptor, JUNO
NCBI Official Symbol
Izumo1r
NCBI Official Synonym Symbols
Juno; Folr4; C86255; Folbp3; 0910001L11Rik
NCBI Protein Information
sperm-egg fusion protein Juno
UniProt Protein Name
Sperm-egg fusion protein Juno
Protein Family
UniProt Gene Name
Izumo1r
UniProt Synonym Gene Names
FR-delta
Uniprot Description
Receptor for IZUMO1 present at the cell surface of oocytes (oolemma), which is essential for species-specific gamete recognition and fertilization. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Does not bind folate.
Research Articles on Izumo1r
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Product Notes
The Izumo1r izumo1r (Catalog #AAA668985) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to mFolate Receptor 4 (Clone: TH6) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's mFolate Receptor 4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: 0.5-1 ug/10^6 . Researchers should empirically determine the suitability of the Izumo1r izumo1r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mFolate Receptor 4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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