Immumohistochemistry (IHC)
(Immunochemical staining of human Mesothelin in human ovarian cancer with mouse monoclonal antibody at 1:60 dilution, formalin-fixed paraffin embedded sections.)
Mouse anti-Human Mesothelin Monoclonal Antibody | anti-MSLN antibody
Anti-Mesothelin Antibody, Mouse Monoclonal
Reactivity
Human
Applications
ELISA, Immunohistochemistry
Purity
Protein A
Synonyms
Mesothelin; Monoclonal Antibody; Anti-Mesothelin Antibody; Mouse Monoclonal; Mesothelin/MSLN Antibody; Mouse MAb; Anti-MPF Antibody; Anti-SMRP Antibody; mesothelin; anti-MSLN antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2a
Clone Number
18
Specificity
Human Mesothelin/MSLN
Purity/Purification
Protein A
Form/Format
Liquid. 0.2um filtered solution in PBS
Applicable Applications for anti-MSLN antibody
ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
ELISA: 1:1000-1:2000
IHC-P: 1:30-1:100
IHC-P: 1:30-1:100
Immunogen
Recombinant Human Mesothelin/MSLN Protein
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human NKG2D/CD314/KLRK1 (rh NKG2D/CD314/KLRK1; P26718-1; Phe78-Val216). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography and conjugated with horseradish-peroxidase (HRP).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Immumohistochemistry (IHC)
(Immunochemical staining of human Mesothelin in human ovarian cancer with mouse monoclonal antibody at 1:60 dilution, formalin-fixed paraffin embedded sections.)
Immumohistochemistry (IHC)
(Immunochemical staining of human Mesothelin in human ovarian cancer with mouse monoclonal antibody at 1:60 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human Mesothelin in human tonsil with mouse monoclonal antibody at 1:60 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human Mesothelin in human tonsil with mouse monoclonal antibody at 1:60 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-MSLN antibody
Background: The megakaryocyte potentiating factor belongs to the mesothelin family. This family is comprised of several mammalian pre-pro-megakaryocyte potentiating factor precursor (MPF) or mesothelin proteins. Mesothelin is a glycosylphosphatidylinositol-linked glycoprotein highly expressed in mesothelial cells, mesotheliomas, and ovarian cancer, but the biological function of the protein is not known. Megakaryocyte potentiating factor is highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). It interacts with MUC16 and potentiates megakaryocyte colony formation in vitro. Megakaryocyte potentiating factor is secreted by several mesothelioma cell lines and is frequently elevated in the blood of patients with mesothelioma. Measurement of this protein may be useful in following the response of mesothelioma to treatment.
References
Chang MC, et al. (2012) Mesothelin enhances invasion of ovarian cancer by inducing MMP-7 through MAPK/ERK and JNK pathways. Biochem J. 442 (2): 293-302.
Nelson HH, et al. (2011) The relationship between tumor MSLN methylation and serum mesothelin (SMRP) in mesothelioma. Epigenetics. 6 (8): 1029-34.
Bournet B, et al. (2012) Gene expression signature of advanced pancreatic ductal adenocarcinoma using low density array on endoscopic ultrasound-guided fine needle aspiration samples. Pancreatology. 12 (1): 27-34.
Nelson HH, et al. (2011) The relationship between tumor MSLN methylation and serum mesothelin (SMRP) in mesothelioma. Epigenetics. 6 (8): 1029-34.
Bournet B, et al. (2012) Gene expression signature of advanced pancreatic ductal adenocarcinoma using low density array on endoscopic ultrasound-guided fine needle aspiration samples. Pancreatology. 12 (1): 27-34.
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Product Notes
The MSLN (Catalog #AAA8119051) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mesothelin Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Mesothelin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). ELISA: 1:1000-1:2000 IHC-P: 1:30-1:100. Researchers should empirically determine the suitability of the MSLN for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mesothelin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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