Mouse MDM2 Monoclonal Antibody | anti-MDM2 antibody

Mouse Monoclonal [clone SMP14] (IgG1) to Human MDM2

Cat. Num. AAA245012

• Gene Names: MDM2; HDMX; hdm2; ACTFS
• Reactivity: Gibbon, Dog, Human, Monkey, Orangutan, Rat; Predicted Reactivity: Bat, Bovine, Hamster, Horse, Pig (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, Immunoprecipitation
• Purity: Purified IgG
• Synonyms: MDM2; Monoclonal Antibody; Mouse Monoclonal [clone SMP14] (IgG1) to Human MDM2; Anti-MDM2 Antibody (aa154-167; clone SMP14) IHC-plus; ACTFS; Double minute 2 protein; Hdm2; MDM2 variant FB30; HDMX; Oncoprotein Mdm2; p53-binding protein Mdm2; MDM2 variant FB28; Human MDM2; anti-MDM2 antibody

• Host: Mouse
• Reactivity: Gibbon, Dog, Human, Monkey, Orangutan, Rat; Predicted Reactivity: Bat, Bovine, Hamster, Horse, Pig (at least 90% immunogen sequence identity)
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: SMP14
• Specificity: Synthetic peptide, CSRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2
• Purity/Purification: Purified IgG
• Form/Format: PBS, 0.09% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 497

• Applicable Applications for anti-MDM2 antibody: Immunohistochemistry (IHC - Paraffin; IHC - Frozen; IHC - Resin), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: IHC-Fr, IHC-P (10 ug/ml), IHC-R, IP, WB (1:1000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide, CSRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2. Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Rat, Dog, Panda (100%); Bat, Cat, Bovine, Hamster, Elephant, Horse, Pig (93%).
• Immunogen Type: Synthetic peptide
• Immunogen: MDM2 antibody was raised against synthetic peptide, CSRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2. Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Rat, Dog, Panda (100%); Bat, Cat, Bovine, Hamster, Elephant, Horse, Pig (93%); Mouse (86%).
• Antigen Modification: aa154-167
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing. Store undiluted.

Immunohistochemistry (IHC)

(Anti-MDM2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 89993689
• NCBI GeneID: 4193
• NCBI Accession #: NP_002383.2
• NCBI GenBank Nucleotide #: NM_002392.5
• UniProt Accession #: Q00987; Q13226; Q13297; Q13298; Q13299; Q13300; Q13301; Q53XW0; Q71TW9; Q8WYJ1; A6NL51; A8K2S6
• Molecular Weight: 55,991 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase Mdm2 isoform a
• NCBI Official Synonym Full Names: MDM2 proto-oncogene, E3 ubiquitin protein ligase
• NCBI Official Symbol: MDM2
• NCBI Official Synonym Symbols: HDMX; hdm2; ACTFS
• NCBI Protein Information: E3 ubiquitin-protein ligase Mdm2; oncoprotein Mdm2; MDM2 oncogene, E3 ubiquitin protein ligase; Mdm2, p53 E3 ubiquitin protein ligase homolog; double minute 2, human homolog of; p53-binding protein; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein
• UniProt Protein Name: E3 ubiquitin-protein ligase Mdm2
• Protein Family: Mdm2-binding protein
• UniProt Gene Name: MDM2
• UniProt Synonym Gene Names: Hdm2
• UniProt Entry Name: MDM2_HUMAN

NCBI Description

This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013]

Uniprot Description

MDM2: a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 via Akt-mediated MDM2 phosphorylation. Phosphorylation of MDM2 increases its interaction with p300, providing a platform to allow the assembly of the protein complex necessary for MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. Facilitates the nuclear export of p53 and targets it for proteasome-mediated proteolysis. Eight alternatively spliced isoforms have been reported.

Protein type: Ubiquitin conjugating system; EC 6.3.2.19; EC 6.3.2.-; Ubiquitin ligase; Nucleolus; Ligase; Oncoprotein; Inhibitor

Chromosomal Location of Human Ortholog: 12q14.3-q15

Cellular Component: nucleoplasm; nuclear body; protein complex; cytoplasm; nucleolus; plasma membrane; synapse; nucleus; cytosol

Molecular Function: identical protein binding; peroxisome proliferator activated receptor binding; protein binding; enzyme binding; zinc ion binding; p53 binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: viral reproduction; nerve growth factor receptor signaling pathway; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination; response to morphine; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; positive regulation of mitotic cell cycle; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; response to carbohydrate stimulus; positive regulation of protein export from nucleus; response to antibiotic; synaptic transmission; negative regulation of DNA damage response, signal transduction by p53 class mediator; response to magnesium ion; establishment of protein localization; peptidyl-lysine modification; positive regulation of cell proliferation; protein complex assembly; response to iron ion; epidermal growth factor receptor signaling pathway; response to drug; traversing start control point of mitotic cell cycle; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; protein destabilization; response to ether; response to cocaine; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; innate immune response; regulation of protein catabolic process; negative regulation of transcription, DNA-dependent

Disease: Accelerated Tumor Formation, Susceptibility To