Mouse anti-Human, Rat Matrix Metalloproteinase 9, 92kD Monoclonal Antibody | anti-MMP9 antibody

Matrix Metalloproteinase 9, 92kD (BSA, Azide Free) (MMP-9, Gelatinase B, Collagenase 4)

Cat. Num. AAA6013187

• Gene Names: MMP9; GELB; CLG4B; MMP-9; MANDP2
• Reactivity: Human, Rat
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Matrix Metalloproteinase 9; 92kD; Monoclonal Antibody; 92kD (BSA; Azide Free) (MMP-9; Gelatinase B; Collagenase 4); Anti -Matrix Metalloproteinase 9; anti-MMP9 antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 0.N.440
• Specificity: Recognizes pro (latent) and activated forms of matrix metalloproteinase 9. Species Crossreactivity: Human and rat.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4. Also supplied with BSA and sodium azide. See M2425-08.

• Applicable Applications for anti-MMP9 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation. Not suitable for use in Immunohistochemistry.; Dilution: Western Blot: 1-2ug/ml for 2 hrs at RT.; Immunoprecipitation: Native.; Optimal dilution determined by the researcher.
• Immunogen: Human native MMP-9. MW of Antigen: Pro form: 92kD Active form: ~86kD.
• Cellular Localization: Cytoplasmic
• Positive Control: Conditioned, serum-free medium from (TPA-treated) human fibrosarcoma HT-1080 or CCL-4 cells.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

Related Product Information for anti-MMP9 antibody

MMPs are a group of enzymes involved in matrix degradation. They share some important characteristics, such as a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and inhibition by specific proteinase inhibitors known as tissue inhibitors of metalloproteinases (TIMPs). Degradation of the extracellular matrix (ECM) is an essential step in tumor invasion and metastasis. The MMPs each have different substrate specificities within the ECM and are important in its degradation. MMP activity is dependent on the levels of activated MMP and TIMPs. MMP-9 is also known as collagenase IV or gelatinase B.

Product Categories/Family for anti-MMP9 antibody

Antibodies; Abs to Matrix Metalloproteinases

NCBI and Uniprot Product Information

• NCBI GI #: 22532481
• NCBI GeneID: 4318
• UniProt Accession #: P14780; Q3LR70; Q8N725; Q9H4Z1; Q9UCJ9; Q9UCL1; Q9UDK2; B2R7V9
• Molecular Weight: 78,458 Da
• NCBI Official Full Name: matrix metalloproteinase 9 (gelatinase B, 92kD gelatinase, 92kD type IV collagenase)
• NCBI Official Synonym Full Names: matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• NCBI Official Symbol: MMP9
• NCBI Official Synonym Symbols: GELB; CLG4B; MMP-9; MANDP2
• NCBI Protein Information: matrix metalloproteinase-9; 92 kDa gelatinase; type V collagenase; macrophage gelatinase; 92 kDa type IV collagenase; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• UniProt Protein Name: Matrix metalloproteinase-9
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: MMP9
• UniProt Synonym Gene Names: CLG4B; MMP-9; GELB
• UniProt Entry Name: MMP9_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]

Uniprot Description

MMP9: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1. Activated by 4-aminophenylmercuric acetate and phorbol ester. Up-regulated by ARHGEF4, SPATA13 and APC via the JNK signaling pathway in colorectal tumor cells. Produced by normal alveolar macrophages and granulocytes. Inhibited by histatin-3 1/24 (histatin-5). Inhibited by ECM1. Belongs to the peptidase M10A family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Protease; EC 3.4.24.35; Secreted

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: collagen binding; identical protein binding; protein binding; zinc ion binding; metalloendopeptidase activity; endopeptidase activity

Biological Process: positive regulation of keratinocyte migration; axon guidance; extracellular matrix disassembly; collagen catabolic process; ossification; extracellular matrix organization and biogenesis; macrophage differentiation; positive regulation of apoptosis; ephrin receptor signaling pathway; proteolysis; skeletal development; leukocyte migration; embryo implantation

Disease: Metaphyseal Anadysplasia 2