Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human melanoma stained withMelan-A MAb (A103). Note cytoplasmic staining of cells.)
Mouse MART-1 Monoclonal Antibody | anti-MART-1 antibody
MART-1 (Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA, Melan-A) (AP)
Gene Names
MLANA; MART1; MART-1
Reactivity
Canine, Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Protein A/G Affinity Chromatography.
Synonyms
MART-1; Monoclonal Antibody; MART-1 (Antigen LB39-AA; Antigen SK29-AA; Melanoma antigen recognized by T-cells 1; MLAN-A; MLANA; Melan-A) (AP); Antigen LB39-AA; Melan-A; anti-MART-1 antibody
Host
Mouse
Reactivity
Canine, Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
A103
Specificity
Recognizes human MART-1. Species Crossreactivity: mouse, rat, and canine. Others-not known.
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Form/Format
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-MART-1 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC) Frozen/Paraffin, Immunoprecipitation (IP), Western Blot (WB)
Application Notes
IHC-F/P: 0.5-1ug/ml for 30 minutes at RT
IP: 0.5-1ug/500ug protein lysate
IF: 0.5-1ug/ml
WB: 0.5-1ug/ml
Applications are based on unconjugated antibody.
IP: 0.5-1ug/500ug protein lysate
IF: 0.5-1ug/ml
WB: 0.5-1ug/ml
Applications are based on unconjugated antibody.
Immunogen
Recombinant hMART-1 protein. Cellular Localization: Cytoplasmic
Conjugate
AP
Positive Control
SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human melanoma stained withMelan-A MAb (A103). Note cytoplasmic staining of cells.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human melanoma stained withMelan-A MAb (A103). Note cytoplasmic staining of cells.)
Product Categories/Family for anti-MART-1 antibody
References
1. Chen Y-T, et. al. Proc Natl Acad Sci, USA, 1996, 93:5915-19.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,157 Da
NCBI Official Full Name
melanoma antigen recognized by T-cells 1
NCBI Official Synonym Full Names
melan-A
NCBI Official Symbol
MLANA
NCBI Official Synonym Symbols
MART1; MART-1
NCBI Protein Information
melanoma antigen recognized by T-cells 1; antigen LB39-AA; antigen SK29-AA; protein Melan-A
UniProt Protein Name
Melanoma antigen recognized by T-cells 1
UniProt Gene Name
MLANA
UniProt Synonym Gene Names
MART1; MART-1
UniProt Entry Name
MAR1_HUMAN
Uniprot Description
MLANA: Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
Protein type: Cell surface; Membrane protein, integral
Chromosomal Location of Human Ortholog: 9p24.1
Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; integral to plasma membrane; melanosome; trans-Golgi network
Molecular Function: protein binding
Research Articles on MART-1
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Product Notes
The MART-1 mlana (Catalog #AAA6123420) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MART-1 (Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA, Melan-A) (AP) reacts with Canine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MART-1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC) Frozen/Paraffin, Immunoprecipitation (IP), Western Blot (WB). IHC-F/P: 0.5-1ug/ml for 30 minutes at RT IP: 0.5-1ug/500ug protein lysate IF: 0.5-1ug/ml WB: 0.5-1ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MART-1 mlana for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MART-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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