Mouse MAPK11 Monoclonal Antibody | anti-MAPK11 antibody

Mouse monoclonal MAPK11 antibody

Cat. Num. AAA5306702

• Gene Names: MAPK11; P38B; SAPK2; p38-2; PRKM11; SAPK2B; p38Beta; P38BETA2
• Applications: Immunofluorescence, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: MAPK11; Monoclonal Antibody; Mouse monoclonal MAPK11 antibody; MAPK11 antibody; anti-MAPK11 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1C2
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 364

• Applicable Applications for anti-MAPK11 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF: 1:100; WB: 1:2000
• Immunogen: MAPK11 antibody was raised in mouse using a full length recombinant protein of human MAPK11 (NP_002742) produced in HEK293T cells, as the immunogen.
• Cross-Reactivity: Human
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant MAPK11 protein using MAPK11 antibody)

Product Categories/Family for anti-MAPK11 antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 20128774
• NCBI GeneID: 5600
• NCBI Accession #: NP_002742.3
• NCBI GenBank Nucleotide #: NM_002751.6
• UniProt Accession #: Q15759; O00284; O15472; Q2XNF2; A8K730; B0LPG1; B7Z630; E7ETQ1; L7RT27
• Molecular Weight: 23,603 Da
• NCBI Official Full Name: mitogen-activated protein kinase 11
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 11
• NCBI Official Symbol: MAPK11
• NCBI Official Synonym Symbols: P38B; SAPK2; p38-2; PRKM11; SAPK2B; p38Beta; P38BETA2
• NCBI Protein Information: mitogen-activated protein kinase 11
• UniProt Protein Name: Mitogen-activated protein kinase 11
• UniProt Gene Name: MAPK11
• UniProt Synonym Gene Names: PRKM11; SAPK2; SAPK2B; MAP kinase 11; MAPK 11; MAP kinase p38 beta; p38b; SAPK2b

NCBI Description

This gene encodes a member of a family of protein kinases that are involved in the integration of biochemical signals for a wide variety of cellular processes, including cell proliferation, differentiation, transcriptional regulation, and development. The encoded protein can be activated by proinflammatory cytokines and environmental stresses through phosphorylation by mitogen activated protein kinase kinases (MKKs). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

P38B: a proline-directed ser/thr MAP kinase, and one of four p38 kinases that play important roles in cellular responses to inflammatory cytokines, DNA damage, oxidative stress, and some GPCRs, leading to direct activation of transcription factors and of other downstream kinases including MSK1, MSK2, eEF2K, MK2, and PRAK. MSK1 and -2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli. MK2 and -3 control gene expression mostly at the post-transcriptional level. eEF2K is important for the elongation of mRNA during translation. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17, which then cleaves the ectodomain of TGF-alpha family ligands, a process leading to the activation of EGFR signaling and cell proliferation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, CHOPO, p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on 'Ser-10' (H3S10ph) in LPS-stimulated myeloid cells. Interacts directly with HDAC3 interacts directly and selectively to repress ATF2 transcriptional activity, and regulate TNF gene expression in LPS-stimulated cells. Inhibited by SB203580 and pyridinyl-imidazole related compounds.

Protein type: CMGC group; EC 2.7.11.24; Kinase, protein; MAPK family; MAPK/p38 subfamily; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); p38 subfamily

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: cytosol; nucleoplasm

Molecular Function: MAP kinase activity; protein binding; protein serine/threonine kinase activity

Biological Process: activation of MAPK activity; positive regulation of erythrocyte differentiation; positive regulation of muscle cell differentiation; Ras protein signal transduction; regulation of transcription factor activity; response to stress; signal transduction; vascular endothelial growth factor receptor signaling pathway