Mouse anti-Human MAP Kinase 15 Monoclonal Antibody | anti-MAPK15 antibody

MAP Kinase 15 (Mitogen-activated Protein Kinase 15, MAPK 15, MAPK15, Extracellular Signal-regulated Kinase 7, ERK7, ERK-7, Extracellular Signal-regulated Kinase 8, ERK8, ERK-8) (AP)

Cat. Num. AAA6120859

• Gene Names: MAPK15; ERK7; ERK8
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: MAP Kinase 15; Monoclonal Antibody; MAP Kinase 15 (Mitogen-activated Protein Kinase 15; MAPK 15; MAPK15; Extracellular Signal-regulated Kinase 7; ERK7; ERK-7; Extracellular Signal-regulated Kinase 8; ERK8; ERK-8) (AP); anti-MAPK15 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 168CT10.6.6
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-MAPK15 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1:1000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MAPK15 Mab is generated from mouse immunized with MAPK15 recombinant protein.
• Conjugate: AP
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C with the addition of glycerol. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive.

Western Blot (WB)

(MAPK15 Antibody western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MAPK15 antibody detected the MAPK15 protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of MAPK15 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-MAPK15 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 95147356
• NCBI GeneID: 225689
• NCBI Accession #: NP_620590.2
• NCBI GenBank Nucleotide #: NM_139021.2
• UniProt Accession #: Q8TD08; Q2TCF9; Q8N362
• Molecular Weight: 59,832 Da
• NCBI Official Full Name: mitogen-activated protein kinase 15
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 15
• NCBI Official Symbol: MAPK15
• NCBI Official Synonym Symbols: ERK7; ERK8
• NCBI Protein Information: mitogen-activated protein kinase 15; ERK-7; ERK-8; MAPK 15; MAP kinase 15; extracellular regulated kinase 8 delta; extracellular signal regulated kinase 8; extracellular signal-regulated kinase 7; extracellular signal-regulated kinase 8
• UniProt Protein Name: Mitogen-activated protein kinase 15
• UniProt Gene Name: MAPK15
• UniProt Synonym Gene Names: ERK7; ERK8; MAP kinase 15; MAPK 15; ERK-7; ERK-8
• UniProt Entry Name: MK15_HUMAN

Uniprot Description

ERK7: In vitro, phosphorylates MBP. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; CMGC group; MAPK family; Erk7 subfamily

Chromosomal Location of Human Ortholog: -

Cellular Component: extracellular region; intracellular; nucleus

Molecular Function: MAP kinase activity; SH3 domain binding; ATP binding

Biological Process: negative regulation of DNA replication; positive regulation of protein ubiquitination; protein amino acid autophosphorylation; positive regulation of protein catabolic process; MAPKKK cascade; negative regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; response to estradiol stimulus