Mouse anti-Human Mannose receptor Monoclonal Antibody

Mannose receptor, Human, mAb 15-2

Cat. Num. AAA584381

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Synonyms: Mannose receptor; Monoclonal Antibody; Human; mAb 15-2; MRC1; CLEC13D; MMR; anti-Mannose receptor antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)

• Applicable Applications for anti-Mannose receptor antibody: F, Flow Cytometry (FC/FACS), FS, Western Blot (WB)
• Application Notes: Application Notes: F: Tissue sections were fixed in acetone/chloroform. Sections were preincubated with normal rabbit serum (Ref.3).; Application Use: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For ligand-receptor intervention studies dilutions have to be made according to the amounts of mannose receptor to be inactivated.
• Immunogen: Purified human mannose receptor from human placental tissue
• Positive Control: Macrophages
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-Mannose receptor antibody

Product Description: The monoclonal antibody 15-2 recognizes the mannose receptor (MR), also known as CD206, a member of the vertebrate C-type lectin family. The mannose receptor, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 175 kDa single-pass type I transmembrane receptor consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail.
The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR binds high-mannose structures on- a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator.
MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells. The expression of MR on monocytes increases during culture and can be enhanced by cytokines as IFN-gamma. Labeling of MR expressing monocytes/macrophages increases with prolonged incubation time probably due to internalization of the MR-antibody-complex. The monoclonal antibody 15-2 prevents binding of glycoproteins including t-PA to MR.
Detection of the MR with anti-MR monoclonal antibody 15-2 can substitute staining for mannose containing probes as labeled mannosylated BSA, a technique which is more cumbersome and less specific.

References

1. Barret-Berghoeff, M et al; Monoclonal antibodies against the human mannose receptor that inhibit the binding of tissue-type plasminogen activator. Thromb Haemostas 1997, 77: 718 2. Noorman, F et al; Monoclonal antibodies against the human mannose receptor as a specific marker in flow cytometry and immunohistochemistry for macrophages. J Leukocyte Biol 1997, 61: 63
3. Noorman, F et al; Cluster Mannosides can inhibit mannose receptor-mediated tissue-type plasminogen activator degradation by both rat and human cells. Hepatology 1997, 26: 1303