Mouse anti-Human MAGEA2 Monoclonal Antibody | anti-MAGEA2 antibody

MAGEA2 (Melanoma-associated Antigen 2, MAGE-2 Antigen, Cancer/Testis Antigen 1.2, CT1.2, MAGE2, MAGEA2A, MGC131923) (FITC)

Cat. Num. AAA6148088

• Gene Names: MAGEA2B; CT1.2; MAGE2
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MAGEA2; Monoclonal Antibody; MAGEA2 (Melanoma-associated Antigen 2; MAGE-2 Antigen; Cancer/Testis Antigen 1.2; CT1.2; MAGE2; MAGEA2A; MGC131923) (FITC); anti-MAGEA2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1H4
• Specificity: Recognizes human MAGEA2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
• Sequence Length: 1817

• Applicable Applications for anti-MAGEA2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-314 from human MAGEA2 (AAH63681) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MPLEQRSQHCKPEEGLEARGEALGLVGAQAPATEEQQTASSSSTLVEVTLGEVPAADSPSPPHSPQGASSFSTTINYTLWRQSDEGSSNQEEEGPRMFPDLESEFQAAISRKMVELVHFLLLKYQAREPVTKAEMLESVLRNCQDFFPVIFSKASEYLQLVFGIEVVEVVPISHLYILVTCLGLSYDGLLGDNQVMPKTGLLIIVLAIIAIEGDCAPEEKIWEELSMLEVFEGREDSVFAHPRKLLMQDLVQENYLEYRQVPGSDPACYEFLWGPRALIETSYVKVLHHTLKIGGEPHISYPPLHERALREGEE
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (60.28kD).)

Western Blot (WB)

(MAGEA2 monoclonal antibody Western Blot analysis of MAGEA2 expression in Jurkat.)

Western Blot (WB)

(Western Blot analysis of MAGEA2 expression in transfected 293T cell line by MAGEA2 monoclonal antibody. Lane 1: MAGEA2 transfected lysate (35kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-MAGEA2 antibody

MAGEA2 is a member of the MAGEA gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 80% sequence identity between each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are expressed at a high level in a number of tumors of various histologic types, and are silent in normal tissues with the exception of testis and placenta. The MAGEA genes are clustered on chromosome Xq28. They may be implicated in some hereditary disorders, such as dyskeratosis congenita. This gene has two identical copies at different loci.

Product Categories/Family for anti-MAGEA2 antibody

Antibodies; Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 39645703
• NCBI GeneID: 266740
• NCBI Official Full Name: Homo sapiens melanoma antigen family A, 2B, mRNA
• NCBI Official Synonym Full Names: MAGE family member A2B
• NCBI Official Symbol: MAGEA2B
• NCBI Official Synonym Symbols: CT1.2; MAGE2
• NCBI Protein Information: melanoma-associated antigen 2
• Protein Family: Melanoma-associated antigen

NCBI Description

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This gene has two identical copies at different loci. [provided by RefSeq, Jul 2008]