Mouse anti-Human MAdCAM-1 Monoclonal Antibody | anti-MAdCAM-1 antibody

MAdCAM-1, Human, mAb 314G8

Cat. Num. AAA584148

• Gene Names: MADCAM1; MACAM1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Synonyms: MAdCAM-1; Monoclonal Antibody; Human; mAb 314G8; anti-MAdCAM-1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 382

• Applicable Applications for anti-MAdCAM-1 antibody: F, Flow Cytometry (FC/FACS), FS, Immunoassay (IA), P, Western Blot (WB)
• Application Notes: Application Use: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of MAdCAM-1 to be inactivated.
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-MAdCAM-1 antibody

Product Description: The monocolonal antibody 314G8 reacts with human mucosal addressin cell adhesion molecules-1 (MAdCAM-1), a key player in mediating the infiltration of leukocytes into chronically inflamed tissue. MAdCAM-1 is a cell-surface Ig superfamily member composed of two extracellular Ig domains, followed by a mucin-like domain, a transmembrane domain and a short cytoplasmatic domain. It interacts via its N-terminal Ig domain with the lymphocyte homing receptor alpha4beta7, which plays a critical role in forming the gut-associated lymphoid system. MAdCAM-1 promotes the adhesion of T- and B cells, monocytes/macrophages, and potentially eosinophils, basophils, and differentiated mast cells to the vascular endothelium. Mucosal addressin cell adhesion molecule-1 RNA transcripts are predominantly expressed in the small intestine, mesenteric lymph nodes, colon and spleen; and are very weakly expresssed in human pancreas and brain. The monocolonal antibody 314G8 recognizes a site in the N-terminal Ig domain of MAdCAM-1. The monoclonal antibody 314G8 detects MAdCAM-1 on venules in the spleen and small intestine. MAdCAM-1 is strongly expressed in the synovium of osteoarthritis patients, predominantly on the endothelial lining of blood vessels, but also within the vessel lumen. The monoclonal antibody 314G8 may be useful in identification of inflammation in humans by monitoring the presence and levels of MAdCAM-1.

References

Leung, E et al; Bioassay detects soluble MAdCAM-1 in body fluids. Immunol Cell Biol 2004, 82: 400

NCBI and Uniprot Product Information

• NCBI GI #: 109633022
• NCBI GeneID: 8174
• NCBI Accession #: NP_570116.2
• NCBI GenBank Nucleotide #: NM_130760.2
• Molecular Weight: 21,247 Da
• NCBI Official Full Name: mucosal addressin cell adhesion molecule 1 isoform a
• NCBI Official Synonym Full Names: mucosal vascular addressin cell adhesion molecule 1
• NCBI Official Symbol: MADCAM1
• NCBI Official Synonym Symbols: MACAM1
• NCBI Protein Information: mucosal addressin cell adhesion molecule 1; MAdCAM-1; hMAdCAM-1; mucosal addressin cell adhesion molecule-1
• UniProt Protein Name: Mucosal addressin cell adhesion molecule 1
• UniProt Gene Name: MADCAM1
• UniProt Synonym Gene Names: MAdCAM-1; hMAdCAM-1
• UniProt Entry Name: MADCA_HUMAN

NCBI Description

The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

MADCAM1: Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L- selectin binding. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: membrane; plasma membrane; integral to membrane

Biological Process: integrin-mediated signaling pathway; regulation of immune response; extracellular matrix organization and biogenesis; cell-matrix adhesion; positive regulation of leukocyte migration; signal transduction; receptor clustering; keratinocyte differentiation; heterotypic cell-cell adhesion; immune response; leukocyte tethering or rolling; cell adhesion; aging