Immunohistochemistry (IHC)
Mouse anti-Human Mad2L1 Monoclonal Antibody | anti-Mad2L1 antibody
Mouse Anti-Mad2L1 [17D10]
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Protein A
Synonyms
Mad2L1; Monoclonal Antibody; Mouse Anti-Mad2L1 [17D10]; Homolog of MAD2 S cerevisiae antibody; HsMAD 2 antibody; HsMAD2 antibody; Human mitotic feedback controlprotein Madp2 antibody; MAD 2 antibody; MAD 2A antibody; MAD2 antibody; MAD2 like 1 antibody; MAD2 like protein1 antibody; MAD2 mitotic arrest deficient like 1 antibody; MAD2A antibody; Mitotic arrest deficient like 1antibody; Mitotic arrest deficient yeast homolog antibody; Mitotic arrest deficient yeast homolog like 1antibody; Mitotic spindle assembly checkpoint protein antibody; anti-Mad2L1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
17D10
Specificity
Western blot with a variety of human cell lines (eg HeLa) confirms that the antibody is specific for Mad2L1.
Purity/Purification
Protein A
Form/Format
Purified antibody (from supernatant) containing PBS 0.1% sodium azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Mad2L1 antibody
Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Optimal antibody dilution should be determined by titration, however as a guideline try; WB: Use a concentration of 2 ug/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 24.6 kDa). IP: Use a concentration of 5 ug/ml
Target Antigen
Mad2L1
Immunogen
Recombinant human Mad2L1
Research Areas
Cell Biology; Cell Division; Nucleus; RNA binding; Spindle; Subcellular Markers; Tags Cell Markers
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
References
Gysin S et al. Analysis of mRNA profiles after MEK1/2 inhibition in human pancreatic cancer cell lines reveals pathways involved in drug sensitivity. Mol Cancer Res 10:1607-19 (2012).WB ; HumanPUBMED Burum-Auensen E et al. Spindle proteins are differentially expressed in the various histological subtypes of testicular germ cell tumors.J Carcinog. 2010 Mar 4;9:1. doi: 10.4103/1477-3163.60358. IHC WB ; HumanPMID: 20411023 Ingemarsdotter C et al. The DNA damage response to non-replicating adeno-associated virus: Centriole overduplication and mitotic catastrophe independent of the spindle checkpoint.Virology. 2010 May 10;400(2):271-86. doi: 10.1016/j.virol.2010.02.003. Epub 2010 Mar 2.PWB ; Human PMID: 20199789 Sarfraz S et al. Modulations of cell cycle checkpoints during HCV associated disease. BMC Infect Dis 9:125 (2009). WB ; Human PUBMED Burum-Auensen E et al. Spindle proteins Aurora A and BUB1B, but not Mad2, are aberrantly expressed in dysplastic mucosa of patients with longstanding ulcerative colitis.J Clin Pathol. 2007 Dec;60(12):1403-8. Epub 2007 Feb 23. IHC ; Human PMID: 17322345 Burum-Auensen E et al. Subcellular localization of the spindle proteins Aurora A, Mad2, and BUBR1 assessed by immunohistochemistry.J Histochem Cytochem. 2007 May;55(5):477-86. Epub 2007 Jan 22.IHC WB ; Human PMID: 17242465 Wise SS et al. Chronic exposure to particulate chromate induces spindle assembly checkpoint bypass in human lung cells. Chem Res Toxicol 19:1492-8 (2006). WB ; Human PUBMED
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Product Notes
The Mad2L1 (Catalog #AAA375206) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Mad2L1 [17D10] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Mad2L1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB). Optimal antibody dilution should be determined by titration, however as a guideline try; WB: Use a concentration of 2 ug/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 24.6 kDa). IP: Use a concentration of 5 ug/ml. Researchers should empirically determine the suitability of the Mad2L1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mad2L1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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