Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Paraffin-embedded Parkinson's diseased vascular brain (Fig.1) and human normal placenta (Fig. 2) tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:300 dilution of MBS631547. Reactivity was detected using an IHC Detection Kit. Staining pattern appears in vascular tissue (Fig. 1 and Fig. 2) and lewy bodies (Fig. 1).)
Mouse anti-Human LRRK2 Monoclonal Antibody | anti-LRRK2 antibody
LRRK2 (Leucine-rich Repeat Kinase 2, RIP7, PARK8, Dardarin)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: Immunohistochemistry: 1:300
Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Paraffin-embedded Parkinson's diseased vascular brain (Fig.1) and human normal placenta (Fig. 2) tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:300 dilution of MBS631547. Reactivity was detected using an IHC Detection Kit. Staining pattern appears in vascular tissue (Fig. 1 and Fig. 2) and lewy bodies (Fig. 1).)
Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Paraffin-embedded Parkinson's diseased vascular brain (Fig.1) and human normal placenta (Fig. 2) tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:300 dilution of MBS631547. Reactivity was detected using an IHC Detection Kit. Staining pattern appears in vascular tissue (Fig. 1 and Fig. 2) and lewy bodies (Fig. 1).)
Western Blot (WB)
(Western Blot Analysis:|Immunoblot versus rat brain low-speed pellet (RBLSP), mouse brain membranes (MBM) and WT and LRRK2 KO mouse samples probed with MBS631547. (UC Davis/NIH NeuroMab Facility, www.neuromab.org, [email protected]))
Western Blot (WB)
(Western Blot Analysis:|Immunoblot versus rat brain low-speed pellet (RBLSP), mouse brain membranes (MBM) and WT and LRRK2 KO mouse samples probed with MBS631547. (UC Davis/NIH NeuroMab Facility, www.neuromab.org, [email protected]))
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. [provided by RefSeq, Jul 2008]
Uniprot Description
LRRK2: a large multidomain protein kinase with a TKL-type kinase domain, multiple protein-protein interaction domains and a mitochondrial Rho domain (MIRO). May play a role in the etiology of Parkinson disease. May also have GTPase activity. Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Interacts with PARK2, PRDX3 and TPCN2. Expressed throughout the adult brain, but at a lower level than in heart and liver. Expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas. Defects in LRRK2 are the cause of Parkinson disease type 8 (PARK8). A slowly progressive neurodegenerative disorder characterized by bradykinesia, rigidity, resting tremor, postural instability, neuronal loss in the substantia nigra, and the presence of neurofibrillary MAPT (tau)-positive and Lewy bodies in some patients.
Protein type: Kinase, protein; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; TKL group; LRRK family
Chromosomal Location of Human Ortholog: 12q12
Cellular Component: dendrite cytoplasm; Golgi apparatus; extracellular space; mitochondrion; lysosome; dendrite; terminal button; inclusion body; trans-Golgi network; cytosol; lipid raft; mitochondrial outer membrane; cell soma; mitochondrial matrix; cytoplasm; mitochondrial inner membrane; cytoplasmic vesicle; endosome; neuron projection; synaptic vesicle membrane; endoplasmic reticulum; perikaryon; axon; mitochondrial membrane; plasma membrane; cell junction
Molecular Function: GTPase activity; tubulin binding; identical protein binding; GTP-dependent protein kinase activity; clathrin binding; protein homodimerization activity; Rho GTPase binding; GTP binding; actin binding; GTPase activator activity; protein kinase activity; MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; SNARE binding; protein kinase A binding; syntaxin-1 binding; kinase activity; glycoprotein binding; ATP binding
Biological Process: regulation of synaptic transmission, glutamatergic; positive regulation of protein binding; activation of MAPKK activity; activation of MAPK activity; protein amino acid autophosphorylation; regulation of neuron maturation; determination of adult life span; protein amino acid phosphorylation; positive regulation of MAP kinase activity; tangential migration from the subventricular zone to the olfactory bulb; negative regulation of protein amino acid phosphorylation; regulation of dopamine receptor signaling pathway; small GTPase mediated signal transduction; negative regulation of protein binding; regulation of mitochondrial depolarization; positive regulation of autophagy; neuromuscular junction development; positive regulation of dopamine receptor signaling pathway; olfactory bulb development; MAPKKK cascade; peptidyl-threonine phosphorylation; endocytosis; regulation of locomotion; positive regulation of programmed cell death; peptidyl-serine phosphorylation; regulation of membrane potential; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; intracellular distribution of mitochondria; autophagy; positive regulation of protein amino acid phosphorylation; response to oxidative stress; regulation of excitatory postsynaptic membrane potential; neurite morphogenesis; positive regulation of GTPase activity
Disease: Parkinson Disease 8, Autosomal Dominant
Research Articles on LRRK2
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Product Notes
The LRRK2 lrrk2 (Catalog #AAA631547) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The LRRK2 (Leucine-rich Repeat Kinase 2, RIP7, PARK8, Dardarin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LRRK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Immunohistochemistry: 1:300. Researchers should empirically determine the suitability of the LRRK2 lrrk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LRRK2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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