Immunoprecipitation (IP)
(Immunoprecipitation of LKB1 using MBS605267: |Lane 1: insulin-treated 293T cell extracts |Lane 2: No-antibody control|Lane 3: Input control)
Rabbit LKB 1 Monoclonal Antibody
LKB 1 (STK11, Serine/Threonine Kinase 11, LKB1, PJS, Renal carcinoma antigen NY-REN-19, Serine/threonine-protein kinase 11, Serine/threonine-protein kinase LKB1)
Reactivity
Human, Monkey, Rat
Applications
Western Blot, Immunoprecipitation
Purity
SupernatantSupernatant
Synonyms
LKB 1; Monoclonal Antibody; LKB 1 (STK11; Serine/Threonine Kinase 11; LKB1; PJS; Renal carcinoma antigen NY-REN-19; Serine/threonine-protein kinase 11; Serine/threonine-protein kinase LKB1); Anti -LKB 1 (STK11; anti-LKB 1 antibody
Host
Rabbit
Reactivity
Human, Monkey, Rat
Clonality
Monoclonal
Isotype
IgG
Clone Number
7H36
Specificity
Recognizes endogenous levels of human LKB1 protein at ~54kD. Species Crossreactivity: Monkey and rat.
Purity/Purification
Supernatant
Supernatant
Supernatant
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Applicable Applications for anti-LKB 1 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Immunoprecipitation and Western Blot.
Dilution: Immunoprecipitation: 1:100
Western Blot: 1:1000
Dilution: Immunoprecipitation: 1:100
Western Blot: 1:1000
Immunogen
Synthetic peptide derived from the sequence of human LKB1, conjugated to KLH.
Positive Control
Caki-1, COS, and insulin treated 293T cell extracts.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunoprecipitation (IP)
(Immunoprecipitation of LKB1 using MBS605267: |Lane 1: insulin-treated 293T cell extracts |Lane 2: No-antibody control|Lane 3: Input control)
Immunoprecipitation (IP)
(Immunoprecipitation of LKB1 using MBS605267: |Lane 1: insulin-treated 293T cell extracts |Lane 2: No-antibody control|Lane 3: Input control)
Western Blot (WB)
(Western Blot analysis of Caki-1 and COS cell extracts using MBS605267.)
Western Blot (WB)
(Western Blot analysis of Caki-1 and COS cell extracts using MBS605267.)
Related Product Information for anti-LKB 1 antibody
LKB1 (STK11) is a serine/threonine kinase and tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases (1,2). A cytosolic protein complex comprised of LKB1, putative kinase STRAD, and the MO25 scaffold protein, activates both AMP-activated protein kinase (AMPK) and several AMPK-related kinases (3). AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations (4). LKB1 appears to be phosphorylated in cells at several sites, including human LKB1 at Ser31/325/428 and Thr189/336/363 (5).
Product Categories/Family for anti-LKB 1 antibody
Similar Products
Product Notes
The LKB 1 (Catalog #AAA605267) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LKB 1 (STK11, Serine/Threonine Kinase 11, LKB1, PJS, Renal carcinoma antigen NY-REN-19, Serine/threonine-protein kinase 11, Serine/threonine-protein kinase LKB1) reacts with Human, Monkey, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LKB 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Immunoprecipitation and Western Blot. Dilution: Immunoprecipitation: 1:100 Western Blot: 1:1000. Researchers should empirically determine the suitability of the LKB 1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LKB 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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