Mouse LFNG Monoclonal Antibody | anti-LFNG antibody

LFNG (LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase, SCDO3) (FITC)

Cat. Num. AAA6174872

• Gene Names: LFNG; SCDO3
• Applications: ELISA, Immunoprecipitation
• Purity: Purified
• Synonyms: LFNG; Monoclonal Antibody; LFNG (LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase; SCDO3) (FITC); LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase; SCDO3; anti-LFNG antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 4F8
• Specificity: Recognizes LFNG.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
• Sequence Length: 250

• Applicable Applications for anti-LFNG antibody: ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: LFNG (AAH14851, 1aa-250aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MTPGRCCLAADIQVETFIFTDGEDEALARHTGNVVITNCSAAHSRQALSCKMAVEYDRFIESGRKWFCHVDDDNYVNLRALLRLLASYPHTRDVYVGKPSLDRPIQAMERVSENKVRPVHFWFATGGAGFCISRGLALKMSPWASGGHFMNTAERIRLPDDCTIGYIVEALLGVPLIRSGLFHSHLENLQQVPTSELHEQVTLSYGMFENKRNAVHVKGPFSVEADPSRFRSIHCHLYPDTPWCPRTAIF
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.; ; FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunoprecipitation (IP)

(Immunoprecipitation of LFNG transfected lysate using anti-LFNG monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with LFNG MaxPab rabbit polyclonal antibody.)

Related Product Information for anti-LFNG antibody

This gene encodes a member of the glycosyltransferase superfamily. The encoded protein is a single-pass type II Golgi membrane protein that functions as a fucose-specific glycosyltransferase, adding an N-acetylglucosamine to the fucose residue of a group of signaling receptors involved in regulating cell fate decisions during development. Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3. Alternatively spliced transcript variants that encode different isoforms have been described, however, not all variants have been fully characterized. [provided by RefSeq]

Product Categories/Family for anti-LFNG antibody

Antibodies; Enzymes; Glycosyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 15990390
• NCBI GeneID: 3955
• NCBI Official Full Name: LFNG protein
• NCBI Official Synonym Full Names: LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
• NCBI Official Symbol: LFNG
• NCBI Official Synonym Symbols: SCDO3
• NCBI Protein Information: beta-1,3-N-acetylglucosaminyltransferase lunatic fringe
• Protein Family: Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe

NCBI Description

This gene is a member of the glycosyltransferase 31 gene family. Members of this gene family, which also includes the MFNG (GeneID: 4242) and RFNG (GeneID: 5986) genes, encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, these proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. The protein encoded by this gene is predicted to be a single-pass type II Golgi membrane protein but it may also be secreted and proteolytically processed like the related proteins in mouse and Drosophila (PMID: 9187150). Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3. [provided by RefSeq, May 2018]