anti-Human Leptin Clone 8F7-A10 Monoclonal Antibody | anti-Lep antibody
Anti-human Leptin (131-145) Monoclonal Antibody Clone 8F7-A10
Gene Names
Lep; OB; obese
Reactivity
Human
Applications
Immunoblot, Western Blot, Western Blot
Synonyms
Leptin Clone 8F7-A10; Monoclonal Antibody; Anti-human Leptin (131-145) Monoclonal Antibody Clone 8F7-A10; anti-Lep antibody
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG
Clone Number
8F7-A10
Specificity
Polypeptide ::: % Cross Reactivity
Leptin (Human) ::: 100
Leptin (92-145)cyclized ::: 100
Leptin (Human) ::: 100
Leptin (92-145)cyclized ::: 100
Form/Format
Supplied as Culture Supernatant
Sequence Length
167
Applicable Applications for anti-Lep antibody
Western Immunoblot, Western Blot, Immunofluorescent Staining of Cells
Application Notes
Western Blot: Western immunoblots of adipocyte homogenates resulted in a single band being detected at ~ 16 kDa at a dilution of 1:100.
Immunofluorescent: This monoclonal antibody has been found to stain specifically human adipocytes at a 1:50 dilution. The ability of this monoclonal antibody to bind to leptin in adipocytes was examined in cells fixed with neutral buffered formalin. The fixed cells were incubated for 20 min with 4% normal goat serum. reacted for 60 minutes with the diluted mouse monoclonal antibody. and then with FITC-conjugated goat anti-mouse IgG. The immunofluorescent staining pattern was observed using epifluorescense microscopy
Immunofluorescent: This monoclonal antibody has been found to stain specifically human adipocytes at a 1:50 dilution. The ability of this monoclonal antibody to bind to leptin in adipocytes was examined in cells fixed with neutral buffered formalin. The fixed cells were incubated for 20 min with 4% normal goat serum. reacted for 60 minutes with the diluted mouse monoclonal antibody. and then with FITC-conjugated goat anti-mouse IgG. The immunofluorescent staining pattern was observed using epifluorescense microscopy
Western Blot Protocol: 1. After SDS-PAGE on 12% percent gels and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the mouse monoclonal antibody after preparing a 1:100 dilution. Use 2% normal goat serum in TBS/Tween-20. Let the primary antibody bind for 2-4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-mouse IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash for 5 min x 4 in TBS/Tween-20.
7. Develop color using the enhanced DAB reaction.
Immunofluorescent: This monoclonal antibody has been found to stain specifically human adipocytes at a 1:50 dilution. The ability of this monoclonal antibody to bind to leptin in adipocytes was examined in cells fixed with neutral buffered formalin. The fixed cells were incubated for 20 min with 4% normal goat serum. reacted for 60 minutes with the diluted mouse monoclonal antibody. and then with FITC-conjugated goat anti-mouse IgG. The immunofluorescent staining pattern was observed using epifluorescense microscopy
Immunofluorescent: This monoclonal antibody has been found to stain specifically human adipocytes at a 1:50 dilution. The ability of this monoclonal antibody to bind to leptin in adipocytes was examined in cells fixed with neutral buffered formalin. The fixed cells were incubated for 20 min with 4% normal goat serum. reacted for 60 minutes with the diluted mouse monoclonal antibody. and then with FITC-conjugated goat anti-mouse IgG. The immunofluorescent staining pattern was observed using epifluorescense microscopy
Western Blot Protocol: 1. After SDS-PAGE on 12% percent gels and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the mouse monoclonal antibody after preparing a 1:100 dilution. Use 2% normal goat serum in TBS/Tween-20. Let the primary antibody bind for 2-4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-mouse IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash for 5 min x 4 in TBS/Tween-20.
7. Develop color using the enhanced DAB reaction.
Related Product Information for anti-Lep antibody
This culture supernatant contains anti-human leptin monoclonal antibody clone 8F7-A10 raised against synthetic human leptin (131-145) attached to a carrier protein. This monoclonal antibody has been shown to bind to the carboxyl terminal region of the intact protein. This monoclonal antibody has been shownd bind to intact leptin specifically in ELISAs. in western immunoblots and by immunocytochemistry. It has been found to be mouse IgG2A by isotyping
Product Categories/Family for anti-Lep antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16162 Da
NCBI Official Full Name
leptin
NCBI Official Synonym Full Names
leptin
NCBI Official Symbol
Lep
NCBI Official Synonym Symbols
OB; obese
NCBI Protein Information
leptin; obesity factor
UniProt Protein Name
Leptin
Protein Family
UniProt Gene Name
Lep
UniProt Synonym Gene Names
Ob
UniProt Entry Name
LEP_RAT
NCBI Description
involved in regulation of body weight, and may have a role in angiogenesis [RGD]
Uniprot Description
Function: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of Lep may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
Subcellular location: Secreted
Probable.
Sequence similarities: Belongs to the leptin family.
Research Articles on Lep
Similar Products
Product Notes
The Lep lep (Catalog #AAA350030) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-human Leptin (131-145) Monoclonal Antibody Clone 8F7-A10 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Leptin Clone 8F7-A10 can be used in a range of immunoassay formats including, but not limited to, Western Immunoblot, Western Blot, Immunofluorescent Staining of Cells. Western Blot: Western immunoblots of adipocyte homogenates resulted in a single band being detected at ~ 16 kDa at a dilution of 1:100. Immunofluorescent: This monoclonal antibody has been found to stain specifically human adipocytes at a 1:50 dilution. The ability of this monoclonal antibody to bind to leptin in adipocytes was examined in cells fixed with neutral buffered formalin. The fixed cells were incubated for 20 min with 4% normal goat serum. reacted for 60 minutes with the diluted mouse monoclonal antibody. and then with FITC-conjugated goat anti-mouse IgG. The immunofluorescent staining pattern was observed using epifluorescense microscopy Immunofluorescent: This monoclonal antibody has been found to stain specifically human adipocytes at a 1:50 dilution. The ability of this monoclonal antibody to bind to leptin in adipocytes was examined in cells fixed with neutral buffered formalin. The fixed cells were incubated for 20 min with 4% normal goat serum. reacted for 60 minutes with the diluted mouse monoclonal antibody. and then with FITC-conjugated goat anti-mouse IgG. The immunofluorescent staining pattern was observed using epifluorescense microscopy Western Blot Protocol: 1. After SDS-PAGE on 12% percent gels and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum in TBS/Tween-20 buffer. 2. Wash x 2 with TBS/Tween-20. 3. Apply the mouse monoclonal antibody after preparing a 1:100 dilution. Use 2% normal goat serum in TBS/Tween-20. Let the primary antibody bind for 2-4 hours. 4. Wash x 3 with TBS/Tween-20. 5. Apply affinity purified HRP-goat anti-mouse IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques. 6. Wash for 5 min x 4 in TBS/Tween-20. 7. Develop color using the enhanced DAB reaction. Researchers should empirically determine the suitability of the Lep lep for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Leptin Clone 8F7-A10, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
