Mouse Lck, p56 Monoclonal Antibody | anti-LCK antibody

Lck, p56 (Lymphocyte-specific Tyrosine Kinase)

Cat. Num. AAA608309

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Lck; p56; Monoclonal Antibody; p56 (Lymphocyte-specific Tyrosine Kinase); Anti -Lck; anti-LCK antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 0.N.385
• Specificity: Recognizes Lck at 56kD. Species Crossreactivity: Human, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide before the addition of glycerol to 40%. Also available without BSA and azide. See L1565-01X.

• Applicable Applications for anti-LCK antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Immunoprecipitation, Kinase Assay, and Western Blot.; Dilution: Immunoprecipitation: Use Protein A. 2ug antibody/mg protein lysate; Western Blot: 1-2ug/ml for 2 hours at RT.
• Immunogen: Recombinant protein corresponding to aa1-225 of murine p56lck protein. Cellular Localization: Cell membrane
• Positive Control: Jurkat cells
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-LCK antibody

p56lck is a membrane-associated non-receptor protein tyrosine kinase from the Src family of kinases. The phosphorylation status and the activity of p56lck is regulated by the CD45 tyrosine protein phosphatase. p56lck plays a critical role in T cell development and activation. p56lck gene is localized to a site in the genome which undergoes frequent chromosomal abnormalities in lymphomas and neuroblastomas.

Product Categories/Family for anti-LCK antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 14140183
• UniProt Accession #: Q95KR7
• Molecular Weight: 58,253 Da
• NCBI Official Full Name: Lck tyrosine kinase
• UniProt Protein Name: Proto-oncogene tyrosine-protein kinase LCK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: LCK
• UniProt Entry Name: LCK_SAISC

Uniprot Description

Function: Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP

By similarity.

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Enzyme regulation: The relative activities of the inhibitory tyrosine-protein kinase CSK and the activating tyrosine-protein phosphatase PTPRC/CD45 determine the level of LCK activity. These interactions allow rapid and efficient activation of LCK in response to TCR stimulation

By similarity. Ref.1

Subunit structure: Binds to the cytoplasmic domain of cell surface receptors, such as AXL, CD2, CD4, CD5, CD8, CD44, CD45 and CD122. Also binds to effector molecules, such as PI4K, VAV1, RASA1, FYB and to other protein kinases including CDK1, RAF1, ZAP70 and SYK. Binds to phosphatidylinositol 3'-kinase (PI3K) from T-lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of KHDRBS1/p70 through its SH2 domain. Interacts with SQSTM1. Interacts with phosphorylated LIME1. Interacts with CBLB and PTPRH. Interacts with RUNX3. Forms a signaling complex with EPHA1, PTK2B AND PI3-KINASE; upon activation by EFNA1 which may regulate T-lymphocytes migration. Associates with ZAP70 and RHOH; these interactions allow LCK-mediated RHOH and CD3 subunit phosphorylations in the presence of functional ZAP70

By similarity. Interacts with Saimiriine herpesvirus 2 TIP. Ref.1

Subcellular location: Cytoplasm

By similarity. Cell membrane; Lipid-anchor; Cytoplasmic side

By similarity. Note: Present in lipid rafts in an inactive form

By similarity.

Tissue specificity: Expressed specifically in lymphoid cells.

Developmental stage: Levels remain relatively constant throughout T-cell ontogeny.

Domain: The SH2 domain mediates interaction with SQSTM1. Interaction is regulated by Ser-59 phosphorylation

By similarity.

Post-translational modification: Autophosphorylated on Tyr-394, increasing enzymatic activity, this site is dephosphorylated by PTN22. Phosphorylated on Tyr-505 by CSK, decreasing activity. Dephosphorylated by PTPRC/CD45. Dephosphorylation at Tyr-394 by PTPN2 negatively regulates T-cells differentiation

By similarity.Myristoylation is required prior to palmitoylation

By similarity.Palmitoylation regulates subcellular location

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family.Contains 1 protein kinase domain.Contains 1 SH2 domain.Contains 1 SH3 domain.

Caution: LCK seems to be active in all vertebrates, except in squirrel monkey T-cells, in which it is inactivated. The reason seems to be that squirrel monkeys are the natural host for Saimiriine herpesvirus 2, which is able to efficiently transform T-cells through a mechanism involving viral Tip/ host LCK interaction. Its inactivation may a mechanism that specifically counteracts the transformation effects of viral Tip.