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Flow Cytometry (FC/FACS)
(Detection of endogenous human LAG-3 by FACS analysis using MBS633034 (human). Method: Human PBMC were stimulated (B) or not (A) with 1ug/ml of superantigen SEB. After 2 days PBMC were stained with 10ug/ml (1ug/0.5x106 cells) of MBS633034 and analyzed by flow cytometry.)
Mouse anti-Human LAG-3 Monoclonal Antibody
LAG-3 (Lymphocyte Activation Gene-3, FDC Protein, CD223) (Biotin)
Reactivity
Human
Applications
ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
Purity
Highly PurifiedPurified by Protein A Sepharose chromatography. >95% by SDS-PAGE.
Synonyms
LAG-3; Monoclonal Antibody; LAG-3 (Lymphocyte Activation Gene-3; FDC Protein; CD223) (Biotin); Anti -LAG-3 (Lymphocyte Activation Gene-3; anti-LAG-3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6D65
Specificity
Recognizes the 30 aa extra-loop of the first N-terminal D1 domain of human LAG-3.
Purity/Purification
Highly Purified
Purified by Protein A Sepharose chromatography. >95% by SDS-PAGE.
Purified by Protein A Sepharose chromatography. >95% by SDS-PAGE.
Form/Format
Supplied as a liquid in PBS. Labeled with Biotin.
Applicable Applications for anti-LAG-3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
Suitable for use in ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot and Inhibitions. Other aapplications not tested.
Dilution: ELISA: Detection.
Immunohistochemistry (Frozen): 1:150
Immunoprecipitation: 10ug/ml
Western Blot: 5ug/ml
Dilution: ELISA: Detection.
Immunohistochemistry (Frozen): 1:150
Immunoprecipitation: 10ug/ml
Western Blot: 5ug/ml
Immunogen
Synthetic peptide corresponding to 30 aa (GPPAAAPGHPLAPGPHPAAPSSWGPRPRRY) from the first N-terminal D1 domain of human LAG-3 (lymphocyte activation gene-3).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Flow Cytometry (FC/FACS)
(Detection of endogenous human LAG-3 by FACS analysis using MBS633034 (human). Method: Human PBMC were stimulated (B) or not (A) with 1ug/ml of superantigen SEB. After 2 days PBMC were stained with 10ug/ml (1ug/0.5x106 cells) of MBS633034 and analyzed by flow cytometry.)
Flow Cytometry (FC/FACS)
(Detection of endogenous human LAG-3 by FACS analysis using MBS633034 (human). Method: Human PBMC were stimulated (B) or not (A) with 1ug/ml of superantigen SEB. After 2 days PBMC were stained with 10ug/ml (1ug/0.5x106 cells) of MBS633034 and analyzed by flow cytometry.)
Testing Data
(Tumor infiltrating lymphocytes (TILs) express LAG-3 (detected using MBS633034. Method: Freshly dissociated single cell suspensions of renal cell carcinoma TILs are incubated with MAb to LAG-3 ) (FITC) (5ug/ml) and anti-MHC Class II molecules (PE) for 30 min. and washed twice in saline buffer. Additional staining with anti-CD3 allowed a gate analysis of total T cells. The LAG-3 and MHC II profiles of CD3+-gated cells for 3 patients are shown.)
Testing Data
(Tumor infiltrating lymphocytes (TILs) express LAG-3 (detected using MBS633034. Method: Freshly dissociated single cell suspensions of renal cell carcinoma TILs are incubated with MAb to LAG-3 ) (FITC) (5ug/ml) and anti-MHC Class II molecules (PE) for 30 min. and washed twice in saline buffer. Additional staining with anti-CD3 allowed a gate analysis of total T cells. The LAG-3 and MHC II profiles of CD3+-gated cells for 3 patients are shown.)
Flow Cytometry (FC/FACS)
(Detection of endogenous human LAG-3 by FACS analysis MBS633034. Method: Human PBMC were stimulated (B) or not (A) with 1ug/ml of superantigen SEB. After 2 days, PBMC were stained with 10ug/ml (1ug/0.5x106 cells) of MBS633034 and analyzed by flow cytometry.)
Flow Cytometry (FC/FACS)
(Detection of endogenous human LAG-3 by FACS analysis MBS633034. Method: Human PBMC were stimulated (B) or not (A) with 1ug/ml of superantigen SEB. After 2 days, PBMC were stained with 10ug/ml (1ug/0.5x106 cells) of MBS633034 and analyzed by flow cytometry.)
Testing Data
(Expression of LAG-3 on CD4+ and CD8+ subpopulations of tumour infiltrating lymphocytes (TILs) detected with MAb to LAG-3 (FITC).|Method: TILs from a dissociated renal cell carcinoma sample, stained with 5ug/ml MAb to LAG-3 (FITC) and FITC-coupled anti-CD4 or -CD8, are analyzed by a two-colour FACS analysis. Additional staining with anti-CD3 allowed a gate analysis of total T cells. Values indicate percentages in each quadrant.)
Testing Data
(Expression of LAG-3 on CD4+ and CD8+ subpopulations of tumour infiltrating lymphocytes (TILs) detected with MAb to LAG-3 (FITC).|Method: TILs from a dissociated renal cell carcinoma sample, stained with 5ug/ml MAb to LAG-3 (FITC) and FITC-coupled anti-CD4 or -CD8, are analyzed by a two-colour FACS analysis. Additional staining with anti-CD3 allowed a gate analysis of total T cells. Values indicate percentages in each quadrant.)
Testing Data
(Specific inhibition of staining. Method: MBS633034 (10ug/ml) (is preincubated with a specific peptide epitope (208b) or a control tetanus toxoid (TT) peptide at different molarities prior to staining of TILs. Stainined cells are then analyzed by FACS.)
Testing Data
(Specific inhibition of staining. Method: MBS633034 (10ug/ml) (is preincubated with a specific peptide epitope (208b) or a control tetanus toxoid (TT) peptide at different molarities prior to staining of TILs. Stainined cells are then analyzed by FACS.)
Related Product Information for anti-LAG-3 antibody
LAG3 (LAG3, LAG-3, lymphocyte-activation gene 3, CD223, lymphocyte-activation protein 3) Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4.
Product Categories/Family for anti-LAG-3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
LAG-3 protein
Similar Products
Product Notes
The LAG-3 (Catalog #AAA633034) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The LAG-3 (Lymphocyte Activation Gene-3, FDC Protein, CD223) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LAG-3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for use in ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot and Inhibitions. Other aapplications not tested. Dilution: ELISA: Detection. Immunohistochemistry (Frozen): 1:150 Immunoprecipitation: 10ug/ml Western Blot: 5ug/ml. Researchers should empirically determine the suitability of the LAG-3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LAG-3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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